Short-term stability study of RNA at room temperature

Conny Mathay, Wusheng Yan, Rodrigo Chuaqui, Amy P N Skubitz, Jae Pil Jeon, Ndate Fall, Fay Betsou, Michael Barnes

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks of storage at room temperature. RNA samples in RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to RNA stored at -80°C (the current gold standard for RNA preservation) and with liquid or dried RNA stored at room temperature without additives in this multi-center study. One center prepared all of the RNA samples, and five participating laboratories applied the samples to the matrices and stored them for 2 weeks at room temperature. Samples were shipped to three testing laboratories, where the 336 RNA samples were rehydrated and then analyzed for RNA recovery, purity, and integrity. Parallel RNA quality analyses and real-time PCR analyses were performed at each of the three testing laboratories. Each of the RNA matrices tested was shown to be fit-for-purpose for short-term room temperature storage in terms of total RNA recovery and rRNA integrity. All but one of the matrices was judged to be fit-for-purpose for mRNA integrity when assessed by real-time PCR analysis. In a follow-up study, RNase-contaminated samples were shown to provide accurate real-time PCR results when stored for up to 3.5 months in either RNAshell or RNAstable.

Original languageEnglish (US)
Pages (from-to)532-542
Number of pages11
JournalBiopreservation and Biobanking
Volume10
Issue number6
DOIs
StatePublished - Dec 1 2012

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RNA Stability
RNA
Temperature
Real-Time Polymerase Chain Reaction
Recovery
Testing
Ribonucleases
Stabilization
Messenger RNA

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Mathay, C., Yan, W., Chuaqui, R., Skubitz, A. P. N., Jeon, J. P., Fall, N., ... Barnes, M. (2012). Short-term stability study of RNA at room temperature. Biopreservation and Biobanking, 10(6), 532-542. https://doi.org/10.1089/bio.2012.0030

Short-term stability study of RNA at room temperature. / Mathay, Conny; Yan, Wusheng; Chuaqui, Rodrigo; Skubitz, Amy P N; Jeon, Jae Pil; Fall, Ndate; Betsou, Fay; Barnes, Michael.

In: Biopreservation and Biobanking, Vol. 10, No. 6, 01.12.2012, p. 532-542.

Research output: Contribution to journalArticle

Mathay, C, Yan, W, Chuaqui, R, Skubitz, APN, Jeon, JP, Fall, N, Betsou, F & Barnes, M 2012, 'Short-term stability study of RNA at room temperature', Biopreservation and Biobanking, vol. 10, no. 6, pp. 532-542. https://doi.org/10.1089/bio.2012.0030
Mathay, Conny ; Yan, Wusheng ; Chuaqui, Rodrigo ; Skubitz, Amy P N ; Jeon, Jae Pil ; Fall, Ndate ; Betsou, Fay ; Barnes, Michael. / Short-term stability study of RNA at room temperature. In: Biopreservation and Biobanking. 2012 ; Vol. 10, No. 6. pp. 532-542.
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