Short chain fatty acids stimulate feline colonic smooth muscle contraction

Mark P. Rondeau, Karen Meltzer, Kathryn E. Michel, Catherine M. McManus, Robert J. Washabau

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The effect of short chain fatty acids (SCFA) on feline colonic smooth muscle contraction was evaluated in vitro. Colonic tissue was obtained from seven healthy male and female adult cats and seven healthy male and female kittens. Longitudinal and circular colonic smooth muscle strips from proximal and distal colon were incubated with SCFA (acetate, butyrate and propionate; 1-100 mM). SCFA-induced contractions were compared to responses obtained using maximal concentrations (10-4 M) of acetylcholine (ACh). The calcium dependence of the SCFA response was investigated by incubating with nifedipine (1 μM) or verapamil (1 μM). Acetate, butyrate and propionate elicited isometric stress responses (0.25-1.98× 104 N/ m2) in longitudinal, but not circular, smooth muscle from both the proximal and distal colon of adult cats. Maximal responses were attained at 50 and 100 mM SCFA. Maximal butyrate and propionate responses were 29 and 19% of the maximal ACh response (10-4 M), respectively. Acetate was least effective in stimulating contractile responses. Nifedipine and verapamil abolished all responses. Contractile responses in kittens were similar to those observed in adult cats, but were smaller in amplitude. Results of these studies have shown that SCFA stimulate longitudinal colonic smooth muscle contractions in kittens and adult cats in vitro. These SCFA-induced contractions involve activation of calcium influx. These in vitro findings may account for some of the effects of dietary fiber on feline colonic motility in vivo.

Original languageEnglish (US)
Pages (from-to)167-173
Number of pages7
JournalJournal of Feline Medicine and Surgery
Volume5
Issue number3
DOIs
StatePublished - Jan 1 2003

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Volatile Fatty Acids
muscle contraction
Felidae
short chain fatty acids
Muscle Contraction
smooth muscle
Smooth Muscle
cats
kittens
Butyrates
Propionates
Cats
butyrates
propionates
Acetates
verapamil
acetates
acetylcholine
Nifedipine
Verapamil

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Short chain fatty acids stimulate feline colonic smooth muscle contraction. / Rondeau, Mark P.; Meltzer, Karen; Michel, Kathryn E.; McManus, Catherine M.; Washabau, Robert J.

In: Journal of Feline Medicine and Surgery, Vol. 5, No. 3, 01.01.2003, p. 167-173.

Research output: Contribution to journalArticle

Rondeau, Mark P. ; Meltzer, Karen ; Michel, Kathryn E. ; McManus, Catherine M. ; Washabau, Robert J. / Short chain fatty acids stimulate feline colonic smooth muscle contraction. In: Journal of Feline Medicine and Surgery. 2003 ; Vol. 5, No. 3. pp. 167-173.
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