Short- and long-term repeatability of fatty acid composition of human plasma phospholipids and cholesterol esters

J. Ma, A. R. Folsom, J. H. Eckfeldt, L. Lewis, L. E. Chambless, C. Paton, J. Bensen, D. Posey, T. Goodwin, R. Hayes, A. Howard, D. L. Jones, A. Haire, B. J. Alliston, F. A. Blackburn, C. W. Britt, B. L. Davis, G. Murton, D. Buckingham

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75 Scopus citations

Abstract

We examined short-term and long-term repeatability (reliability) of the fatty acid (FA) composition of plasma phospholipids and cholesterol esters (CEs). For short-term reliability, fasting blood samples of 34 subjects were collected three times, 2 wk apart, and in 24 subjects duplicate samples were collected during each visit. For long-term reliability, two fasting samples were collected in 50 subjects ≃3 y apart. In both phospholipids and CEs, short-term and long-term reliability coefficients were > 0.65 for the major plasma FAs (16:0, 18:0, 18: 2n-6, and 20:4n-6), with the exception of 18:1n- 9, but were generally lower for FAs that compose < 1% of total FAs. Reliability tended to be better for CEs than for phospholipids. Method variability was small (< 5% of total variability for most FAs), indicating that biological and dietary variability contribute most to total variability. Plasma FA measurement warrants consideration as a biochemical marker of diet in epidemiologic studies.

Original languageEnglish (US)
Pages (from-to)572-578
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume62
Issue number3
DOIs
StatePublished - Sep 1995

Keywords

  • Fatty acids
  • cholesterol esters
  • phospholipids
  • plasma
  • reliability

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