Shining new light on 3D cell motility and the metastatic process

Paolo P. Provenzano, Kevin W. Eliceiri, Patricia J. Keely

Research output: Contribution to journalReview article

45 Citations (Scopus)

Abstract

Understanding tissue architecture and physical and chemical reciprocity between cells and their microenvironment provides vital insights into key events in cancer metastasis, such as cell migration through three-dimensional (3D) extracellular matrices. Yet, many mechanistic details associated with metastasis remain elusive due to the difficulty of studying cancer cells in relevant 3D microenvironments. Recently, optical imaging has facilitated the direct observation of single cells as they undertake fundamental steps in the metastatic processes. Optical imaging is also providing novel 'optical biomarkers' with diagnostic potential that are linked to cell-motility pathways associated with metastasis, and these can to help guide new approaches to cancer diagnosis and therapy. Here we present recent advances in one subclass of optical imaging of particular promise for cellular imaging - multiphoton microscopy - that can be used to improve the detection of malignant cells as well as advance our understanding of the cell biology of cancer metastasis.

Original languageEnglish (US)
Pages (from-to)638-648
Number of pages11
JournalTrends in Cell Biology
Volume19
Issue number11
DOIs
StatePublished - Nov 1 2009

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Cell Movement
Optical Imaging
Neoplasm Metastasis
Neoplasms
Cellular Microenvironment
Extracellular Matrix
Cell Biology
Microscopy
Biomarkers
Observation
Therapeutics

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Shining new light on 3D cell motility and the metastatic process. / Provenzano, Paolo P.; Eliceiri, Kevin W.; Keely, Patricia J.

In: Trends in Cell Biology, Vol. 19, No. 11, 01.11.2009, p. 638-648.

Research output: Contribution to journalReview article

Provenzano, Paolo P. ; Eliceiri, Kevin W. ; Keely, Patricia J. / Shining new light on 3D cell motility and the metastatic process. In: Trends in Cell Biology. 2009 ; Vol. 19, No. 11. pp. 638-648.
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