Shifting microbiomes complement life stage transitions and diet of the bird parasite Philornis downsi from the Galapagos Islands

Polpass Arul Jose, Michael Ben-Yosef, Paola Lahuatte, Charlotte E. Causton, George E. Heimpel, Edouard Jurkevitch, Boaz Yuval

Research output: Contribution to journalArticlepeer-review

Abstract

Domestication disconnects an animal from its natural environment and diet, imposing changes in the attendant microbial community. We examine these changes in Philornis downsi (Muscidae), an invasive parasitic fly of land birds in the Galapagos Islands. Using a 16S rDNA profiling approach we studied the microbiome of larvae and adults of wild and laboratory-reared populations. These populations diverged in their microbiomes, significantly more so in larval than in adult flies. In field-collected second-instar larvae, Klebsiella (70.3%) was the most abundant taxon, while in the laboratory Ignatzschineria and Providencia made up 89.2% of the community. In adults, Gilliamella and Dysgonomonas were key members of the core microbiome of field-derived females and males but had no or very low representation in the laboratory. Adult flies harbour sex-specific microbial consortia in their gut, as male core microbiomes were significantly dominated by Klebsiella. Thus, P. downsi microbiomes are dynamic and shift correspondingly with life cycle and diet. Sex-specific foraging behaviour of adult flies and nest conditions, which are absent in the laboratory, may contribute to shaping distinct larval, and adult male and female microbiomes. We discuss these findings in the context of microbe–host co-evolution and the implications for control measures.

Original languageEnglish (US)
Pages (from-to)5014-5029
Number of pages16
JournalEnvironmental microbiology
Volume23
Issue number9
Early online dateFeb 15 2021
DOIs
StatePublished - Sep 2021

Bibliographical note

Funding Information:
This work was funded by a grant from the US-Israel Binational Science Foundation (BSF #2016046). P.A.J. is jointly supported by HUJI and the Planning and Budgeting Committee (PBC) of the Council for Higher Education (Israel). We thank Ainoa Nieto, Irene Pe?a Galindo, Andrea Cahuana, Magally Infante, Shlomit Shloush and Ofra Matan for support during our expeditions; Sabrina McNew and Courtney Pike for providing the nests. Thanks to Jonathan Friedman for his valuable suggestions on microbiome data analysis. Permission to conduct this study was granted by the Galapagos National Park Directorate (Project: PC-35-19 & PC-07-18: Control of the Invasive Parasite, Philornis downsi and its Impact on Biodiversity) and the Ecuadorian Ministry of the Environment (MAE-DNB-CM-2016-0043). This is contribution number 2341 of the Charles Darwin Foundation for the Galapagos Islands.

Funding Information:
This work was funded by a grant from the US‐Israel Binational Science Foundation (BSF #2016046). P.A.J. is jointly supported by HUJI and the Planning and Budgeting Committee (PBC) of the Council for Higher Education (Israel). We thank Ainoa Nieto, Irene Peña Galindo, Andrea Cahuana, Magally Infante, Shlomit Shloush and Ofra Matan for support during our expeditions; Sabrina McNew and Courtney Pike for providing the nests. Thanks to Jonathan Friedman for his valuable suggestions on microbiome data analysis. Permission to conduct this study was granted by the Galapagos National Park Directorate (Project: PC‐35‐19 & PC‐07‐18: Control of the Invasive Parasite, and its Impact on Biodiversity) and the Ecuadorian Ministry of the Environment (MAE‐DNB‐CM‐2016‐0043). This is contribution number 2341 of the Charles Darwin Foundation for the Galapagos Islands. Philornis downsi

Publisher Copyright:
© 2021 Society for Applied Microbiology and John Wiley & Sons Ltd.

PubMed: MeSH publication types

  • Journal Article

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