Shewanella pealeana sp. nov., a member of the microbial community associated with the accessory nidamental gland of the squid Loligo pealei

Michael R. Leonardo, Duane P. Moser, Elena Barbieri, Christine A. Brantner, Barbara J. MacGregor, Bruce J. Paster, Erko Stackebrandt, Kenneth H. Nealson

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Abstract

A new, mesophillic, facultatively anaerobic, psychrotolerant bacterium, strain ANG-SQ1(T) (T = type strain), was isolated from a microbial community colonizing the accessory nidamental gland of the squid Loligo pealei. It was selected from the community on the basis of its ability to reduce elemental sulfur. The cells are motile, Gram-negative rods (2.0-3.0 μm long, 0.4-0.6 μm wide). ANG-SQ1(T) grows optimally over the temperature range of 25-30 °C and a pH range of 6.5-7.5 °C in media containing 0.5 M NaCl. 16S rRNA sequence analysis revealed that this organism belongs to the γ-3 subclass of the Proteobacteria. The closest relative of ANG-SQ1(T) is Shewanella gelidimarina, with a 16S rRNA sequence similarity of 97.0%. Growth occurs with glucose, lactate, acetate, pyruvate, glutamate, citrate, succinate, Casamino acids, yeast extract or peptone as sole energy source under aerobic conditions. The isolate grows anaerobically by the reduction of iron, manganese, nitrate, fumarate, trimethylamine-N-oxide, thiosulfate or elemental sulfur as terminal electron acceptor with lactate. Growth of ANG- SQ1(T) was enhanced by the addition of choline chloride to growth media lacking Casamino acids. The addition of leucine or valine also enhanced growth in minimal growth media supplemented with choline. The results of both phenotypic and genetic characterization indicate that ANG-SQ1T is a Shewanella species. Thus it is proposed that this new isolate be assigned to the genus Shewanella and that it should be named Shewanella pealeana sp. nov., in recognition of its association with L, pealei.

Original languageEnglish (US)
Pages (from-to)1341-1351
Number of pages11
JournalInternational Journal of Systematic Bacteriology
Volume49
Issue number4
DOIs
StatePublished - Oct 1999
Externally publishedYes

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Keywords

  • Choline
  • Loligo pealei
  • Shewanella pealeana
  • Sulfur reducer

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