SERE, a widely dispersed bacterial repetitive DNA element

G. Rajashekara, T. Koeuth, S. Nevile, A. Back, Kakambi V Nagaraja, J. R. Lupski, V. Kapur

Research output: Contribution to journalArticle

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Abstract

The presence of a Salmonella serotype Enteritidis repeat element (SERE) located within the upstream regulatory region of the sefABCD operon encoding fimbrial proteins is reported. DNA dot-blot hybridisation analyses and computerised searches of genetic databases indicate that SERE is well conserved and widely distributed throughout the bacterial and archaeal kingdoms. A SERE-based polymerase chain reaction (SERE-PCR) assay was developed to fingerprint 54 isolates of Enteritidis representing nine distinct phage types and 54 isolates of other Salmonella serotypes. SERE-PCR identified five distinct fingerprint profiles among the 54 Enteritidis isolates; no correlation between phage types and SERE-PCR fingerprint patterns was noticed. SERE-PCR was reproducible, rapid and easy to perform. The results of this investigation suggest that the limited heterogeneity of SERE-PCR fingerprint patterns can be utilised to develop serotype- and serogroup-specific fingerprint patterns for isolates of Enteritidis.

Original languageEnglish (US)
Pages (from-to)489-497
Number of pages9
JournalJournal of medical microbiology
Volume47
Issue number6
DOIs
StatePublished - Jan 1 1998

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    Rajashekara, G., Koeuth, T., Nevile, S., Back, A., Nagaraja, K. V., Lupski, J. R., & Kapur, V. (1998). SERE, a widely dispersed bacterial repetitive DNA element. Journal of medical microbiology, 47(6), 489-497. https://doi.org/10.1099/00222615-47-6-489