Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles

David Garenne, Laure Beven, Laurence Navailles, Frédéric Nallet, Erick J. Dufourc, Jean Paul Douliez

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pH value, the precrowding feature in coacervates allowing for protein encapsulation within vesicles. We then produced enzyme-enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors.

Original languageEnglish (US)
Pages (from-to)13475-13479
Number of pages5
JournalAngewandte Chemie - International Edition
Volume55
Issue number43
DOIs
StatePublished - Jan 1 2016

Keywords

  • coacervates
  • encapsulation
  • microreactors
  • proteins
  • vesicles

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