Abstract
A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 37-39 |
| Number of pages | 3 |
| Journal | Human Genetics |
| Volume | 76 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 1987 |
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