Sequential G- to R-banding for high resolution chromosome analysis

Betsy Hirsch; Rosalie Mack; Diane Arthur

doi:10.1007/BF00283047

Sequential G- to R-banding for high resolution chromosome analysis

Betsy Hirsch, Rosalie Mack, Diane Arthur

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

Original language	English (US)
Pages (from-to)	37-39
Number of pages	3
Journal	Human Genetics
Volume	76
Issue number	1
DOIs	https://doi.org/10.1007/BF00283047
State	Published - May 1 1987

Publisher link

10.1007/BF00283047

Find It

Find It at U of M Libraries

Cite this

@article{a69068f228a647ba9cc51fc38d251397,

title = "Sequential G- to R-banding for high resolution chromosome analysis",

abstract = "A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.",

author = "Betsy Hirsch and Rosalie Mack and Diane Arthur",

year = "1987",

month = may,

day = "1",

doi = "10.1007/BF00283047",

language = "English (US)",

volume = "76",

pages = "37--39",

journal = "Human Genetics",

issn = "0340-6717",

publisher = "Springer Verlag",

number = "1",

}

TY - JOUR

T1 - Sequential G- to R-banding for high resolution chromosome analysis

AU - Hirsch, Betsy

AU - Mack, Rosalie

AU - Arthur, Diane

PY - 1987/5/1

Y1 - 1987/5/1

N2 - A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

AB - A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

UR - http://www.scopus.com/inward/record.url?scp=0023238474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023238474&partnerID=8YFLogxK

U2 - 10.1007/BF00283047

DO - 10.1007/BF00283047

M3 - Article

C2 - 2437009

AN - SCOPUS:0023238474

SN - 0340-6717

VL - 76

SP - 37

EP - 39

JO - Human Genetics

JF - Human Genetics

IS - 1

ER -

Sequential G- to R-banding for high resolution chromosome analysis

Abstract

Publisher link

Find It

Other files and links

Fingerprint

Cite this