Sequence-Specific Nucleases for Genetic Improvement of Potato

Nathaniel M. Butler, David S. Douches

Research output: Contribution to journalReview articlepeer-review

6 Scopus citations

Abstract

Genome editing using sequence-specific nucleases (SSNs) is rapidly becoming a standard tool for genetic engineering in crop species. The implementation of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and CRISPR/Cas (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems (Cas)) for inducing double-strand breaks enables targeting of virtually any sequence for genetic modification. Targeted mutagenesis via nonhomologous end-joining (NHEJ) and gene targeting via homologous recombination (HR) have been demonstrated in a number of plant species but reports have been limited in vegetatively propagated crops, such as potato (Solanum tuberosum Group Tuberosum L.). This review provides a historical overview of genetic engineering in agriculture, applications of SSN technologies for genome editing in plant species, and prospects of using SSNs for genetic improvement of potato.

Original languageEnglish (US)
Pages (from-to)303-320
Number of pages18
JournalAmerican Journal of Potato Research
Volume93
Issue number4
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016, The Potato Association of America.

Keywords

  • CRISPR/Cas
  • Gene targeting
  • Genome editing
  • Homologous recombination
  • TALEN
  • Targeted mutagenesis

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