Sequence-related amplified polymorphisms (Sraps) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China

Jinna Hou, Sufang An, Yongjuan Liu, Li Lei, Baoquan Li

Research output: Contribution to journalArticlepeer-review

Abstract

The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.

Original languageEnglish (US)
Pages (from-to)155-160
Number of pages6
JournalCrop Breeding and Applied Biotechnology
Volume18
Issue number2
DOIs
StatePublished - 2018

Keywords

  • Genetic diversity
  • High efficient molecular marker
  • SRAP
  • Upland cotton

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