Sequence of a mosquito ribonucleotide reductase cDNA and evidence for gene amplification in hydroxyurea-resistant cells

F. A. Shotkoski, G. Jayachandran, G. Stamatoyannopoulos, Ann M. Fallon

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Ribonucleotide reductase is essential for production of the deoxyribonucleotide substrates required for DNA synthesis in all eukaryotic cells. The full-length cDNA encoding a mosquito ribonucleotide reductase R2 subunit was obtained from Aedes albopictus cells using a polymerase chain reaction (PCR)-based approach. The cDNA contained 1197 nucleotides, and encoded a 398 amino acid R2 protein. Overall, mosquito R2 shared approximately 70% amino acid sequence identity with R2 proteins from vertebrates, but conservation at the N-terminus of the protein was relatively low. The sequence of R2 cDNA was identical in hydroxyurea-resistant mosquito cells and in wild-type cells, while the R2 gene copy number was increased ten to twentyfold in resistant cells.

Original languageEnglish (US)
Pages (from-to)565-570
Number of pages6
JournalInsect molecular biology
Volume8
Issue number4
DOIs
StatePublished - Nov 1999

Keywords

  • Gene amplification
  • Hydroxyurea
  • Mosquito cell line
  • Ribonucleotide reductase
  • Transcription

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