Abstract
Avian pneumovirus (APV) has recently been described as the cause of a new respiratory syndrome in turkey flocks in the United States. We here describe the complete sequence of the nucleocapsid (N) and phosphoprotein (P) genes of this emerging APV (APV/US). Our results show 59 and 61% nucleotide sequence identity of the APV/US N gene with N genes of previously described European APV subgroups A and B, respectively. The P gene of APV/US showed only 53% nucleotide sequence identity with the ortholog from APV subgroup A. Phylogenetic analyses of both N and P genes clearly demonstrate that the APV/US lineage is evolutionarily related but distinct from European APVs. Moreover, sequence analysis of the N and P genes from two laboratory adapted isolates of APV/US (APV/MN-1a and APV/MN-1b) and from ten clinical samples from APV-infected turkeys suggests only modest level of amino acid divergence in the N (0-0.3%) and P (0-1.4%) proteins. Taken together, the results of this study indicate that APV/US represents a new subgroup (subgroup C) of APV and show that there is limited heterogeneity in the N and P genes of APV/US isolates.
Original language | English (US) |
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Pages (from-to) | 15-25 |
Number of pages | 11 |
Journal | Virus research |
Volume | 79 |
Issue number | 1-2 |
DOIs | |
State | Published - Oct 5 2001 |
Bibliographical note
Funding Information:Research in the laboratory of V. Kapur is supported in part by National Institute of Health, US Department of Agriculture, Minnesota Agricultural Experiment Station, and Minnesota Turkey Growers Association.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
Keywords
- Avian pneumovirus
- Nucleocapsid gene
- Phosphoprotein gene
- Phylogenetic relationships
- Sequence analysis