Sequence analysis of the nucleocapsid and phosphoprotein genes of avian pneumoviruses circulating in the US

Arshud M. Dar, Shirin Munir, Sagar M. Goyal, Mitchell S. Abrahamsen, Vivek Kapur

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Avian pneumovirus (APV) has recently been described as the cause of a new respiratory syndrome in turkey flocks in the United States. We here describe the complete sequence of the nucleocapsid (N) and phosphoprotein (P) genes of this emerging APV (APV/US). Our results show 59 and 61% nucleotide sequence identity of the APV/US N gene with N genes of previously described European APV subgroups A and B, respectively. The P gene of APV/US showed only 53% nucleotide sequence identity with the ortholog from APV subgroup A. Phylogenetic analyses of both N and P genes clearly demonstrate that the APV/US lineage is evolutionarily related but distinct from European APVs. Moreover, sequence analysis of the N and P genes from two laboratory adapted isolates of APV/US (APV/MN-1a and APV/MN-1b) and from ten clinical samples from APV-infected turkeys suggests only modest level of amino acid divergence in the N (0-0.3%) and P (0-1.4%) proteins. Taken together, the results of this study indicate that APV/US represents a new subgroup (subgroup C) of APV and show that there is limited heterogeneity in the N and P genes of APV/US isolates.

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalVirus research
Issue number1-2
StatePublished - Oct 5 2001

Bibliographical note

Funding Information:
Research in the laboratory of V. Kapur is supported in part by National Institute of Health, US Department of Agriculture, Minnesota Agricultural Experiment Station, and Minnesota Turkey Growers Association.

Copyright 2017 Elsevier B.V., All rights reserved.


  • Avian pneumovirus
  • Nucleocapsid gene
  • Phosphoprotein gene
  • Phylogenetic relationships
  • Sequence analysis


Dive into the research topics of 'Sequence analysis of the nucleocapsid and phosphoprotein genes of avian pneumoviruses circulating in the US'. Together they form a unique fingerprint.

Cite this