Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)

Rashmi Raj; Charanjeet Kaur; Ashish Srivastava; Susheel Kumar; S. K. Raj

doi:10.1007/s13205-020-02418-8

Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)

Rashmi Raj
, Charanjeet Kaur
, Ashish Srivastava
, Susheel Kumar
, S. K. Raj

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CEVA: KF430815, KF430816, KM066973, KM066974); narcissus yellow stripe virus (NYSV: KM066972, JQ686724) and narcissus degeneration virus (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus.

Original language	English (US)
Article number	428
Journal	3 Biotech
Volume	10
Issue number	10
DOIs	https://doi.org/10.1007/s13205-020-02418-8
State	Published - Oct 1 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020, King Abdulaziz City for Science and Technology.

Keywords

CEVA
Leaf yellow stripe
N. tazetta var. paperwhite
NDV
NYSV
Phylogenetic relationships
RT-PCR amplification
Sequence identity

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10.1007/s13205-020-02418-8

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title = "Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)",

abstract = "Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of \textasciitilde{} 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CEVA: KF430815, KF430816, KM066973, KM066974); narcissus yellow stripe virus (NYSV: KM066972, JQ686724) and narcissus degeneration virus (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus.",

keywords = "CEVA, Leaf yellow stripe, N. tazetta var. paperwhite, NDV, NYSV, Phylogenetic relationships, RT-PCR amplification, Sequence identity",

author = "Rashmi Raj and Charanjeet Kaur and Ashish Srivastava and Susheel Kumar and Raj, \{S. K.\}",

note = "Publisher Copyright: {\textcopyright} 2020, King Abdulaziz City for Science and Technology.",

year = "2020",

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day = "1",

doi = "10.1007/s13205-020-02418-8",

language = "English (US)",

volume = "10",

journal = "3 Biotech",

issn = "2190-572X",

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TY - JOUR

T1 - Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)

AU - Raj, Rashmi

AU - Kaur, Charanjeet

AU - Srivastava, Ashish

AU - Kumar, Susheel

AU - Raj, S. K.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CEVA: KF430815, KF430816, KM066973, KM066974); narcissus yellow stripe virus (NYSV: KM066972, JQ686724) and narcissus degeneration virus (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus.

AB - Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CEVA: KF430815, KF430816, KM066973, KM066974); narcissus yellow stripe virus (NYSV: KM066972, JQ686724) and narcissus degeneration virus (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus.

KW - CEVA

KW - Leaf yellow stripe

KW - N. tazetta var. paperwhite

KW - NDV

KW - NYSV

KW - Phylogenetic relationships

KW - RT-PCR amplification

KW - Sequence identity

UR - https://www.scopus.com/pages/publications/85091205613

UR - https://www.scopus.com/pages/publications/85091205613#tab=citedBy

U2 - 10.1007/s13205-020-02418-8

DO - 10.1007/s13205-020-02418-8

M3 - Article

AN - SCOPUS:85091205613

SN - 2190-572X

VL - 10

JO - 3 Biotech

JF - 3 Biotech

IS - 10

M1 - 428

ER -

Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)

Abstract

Bibliographical note

Keywords

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