Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: Potential for emergency diagnosis of inborn errors of metabolism

Olivier Boulat; David G. McLaren; Edgar A. Arriaga; David D Y Chen

doi:10.1016/S0378-4347(00)00611-3

Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: Potential for emergency diagnosis of inborn errors of metabolism

Olivier Boulat, David G. McLaren, Edgar A. Arriaga, David D Y Chen

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Free amino acids (AAs) in human plasma are derivatized with 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) and analyzed by capillary electrophoresis (CE) with laser induced fluorescence (LIF) detection. The labeling procedure is significantly improved over results reported previously. Derivatization can be completed in 40 min, with concentrations as low as 4×10^-8 M successfully labeled in favourable cases. Twenty-nine AAs (including 2 internal standards) are identified and can be reproducibly separated in 70 min. Migration time RSD values for 23 of these AAs were calculated and found in the range from 0.5 to 4%. The rapid derivatization procedure and the resolution obtained in the separation are sufficient for a semi-quantitative, emergency diagnosis of several inborn errors of metabolism (IEM). Amino acid profiles for both normal donor plasma samples and plasma samples of patients suffering from phenylketonuria, tyrosinemia, maple syrup urinary disease, hyperornithinemia, and citrullinemia are studied.

Original language	English (US)
Pages (from-to)	217-228
Number of pages	12
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	754
Issue number	1
DOIs	https://doi.org/10.1016/S0378-4347(00)00611-3
State	Published - Apr 15 2001

Bibliographical note

Funding Information:
This work is supported by the Centre Hospitalier Universitaire Vaudois and the Laboratoire Central de Chimie Clinique of the Centre Hospitalier Universitaire Vaudois (Head: Prof. C. Bachmann) and the Natural Science and Engineering Research Council of Canada. The IEC analyses were performed by F. Huppertz.

Keywords

Amino acids
Inborn errors

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10.1016/S0378-4347(00)00611-3

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Boulat, O., McLaren, D. G., Arriaga, E. A., & Chen, D. D. Y. (2001). Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: Potential for emergency diagnosis of inborn errors of metabolism. Journal of Chromatography B: Biomedical Sciences and Applications, 754(1), 217-228. https://doi.org/10.1016/S0378-4347(00)00611-3

Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: Potential for emergency diagnosis of inborn errors of metabolism. / Boulat, Olivier; McLaren, David G.; Arriaga, Edgar A. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 754, No. 1, 15.04.2001, p. 217-228.

Research output: Contribution to journal › Article › peer-review

Boulat, O, McLaren, DG, Arriaga, EA & Chen, DDY 2001, 'Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: Potential for emergency diagnosis of inborn errors of metabolism', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 754, no. 1, pp. 217-228. https://doi.org/10.1016/S0378-4347(00)00611-3

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abstract = "Free amino acids (AAs) in human plasma are derivatized with 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) and analyzed by capillary electrophoresis (CE) with laser induced fluorescence (LIF) detection. The labeling procedure is significantly improved over results reported previously. Derivatization can be completed in 40 min, with concentrations as low as 4×10-8 M successfully labeled in favourable cases. Twenty-nine AAs (including 2 internal standards) are identified and can be reproducibly separated in 70 min. Migration time RSD values for 23 of these AAs were calculated and found in the range from 0.5 to 4\%. The rapid derivatization procedure and the resolution obtained in the separation are sufficient for a semi-quantitative, emergency diagnosis of several inborn errors of metabolism (IEM). Amino acid profiles for both normal donor plasma samples and plasma samples of patients suffering from phenylketonuria, tyrosinemia, maple syrup urinary disease, hyperornithinemia, and citrullinemia are studied.",

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N2 - Free amino acids (AAs) in human plasma are derivatized with 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) and analyzed by capillary electrophoresis (CE) with laser induced fluorescence (LIF) detection. The labeling procedure is significantly improved over results reported previously. Derivatization can be completed in 40 min, with concentrations as low as 4×10-8 M successfully labeled in favourable cases. Twenty-nine AAs (including 2 internal standards) are identified and can be reproducibly separated in 70 min. Migration time RSD values for 23 of these AAs were calculated and found in the range from 0.5 to 4%. The rapid derivatization procedure and the resolution obtained in the separation are sufficient for a semi-quantitative, emergency diagnosis of several inborn errors of metabolism (IEM). Amino acid profiles for both normal donor plasma samples and plasma samples of patients suffering from phenylketonuria, tyrosinemia, maple syrup urinary disease, hyperornithinemia, and citrullinemia are studied.

AB - Free amino acids (AAs) in human plasma are derivatized with 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) and analyzed by capillary electrophoresis (CE) with laser induced fluorescence (LIF) detection. The labeling procedure is significantly improved over results reported previously. Derivatization can be completed in 40 min, with concentrations as low as 4×10-8 M successfully labeled in favourable cases. Twenty-nine AAs (including 2 internal standards) are identified and can be reproducibly separated in 70 min. Migration time RSD values for 23 of these AAs were calculated and found in the range from 0.5 to 4%. The rapid derivatization procedure and the resolution obtained in the separation are sufficient for a semi-quantitative, emergency diagnosis of several inborn errors of metabolism (IEM). Amino acid profiles for both normal donor plasma samples and plasma samples of patients suffering from phenylketonuria, tyrosinemia, maple syrup urinary disease, hyperornithinemia, and citrullinemia are studied.

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Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: Potential for emergency diagnosis of inborn errors of metabolism

Abstract

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