Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis

Norine Best; Edgar Arriaga; Yong Da Chen; Norman J. Dovichi

doi:10.1021/ac00094a031

Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis

Norine Best
, Edgar Arriaga
, Yong Da Chen
, Norman J. Dovichi

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.

Original language	English (US)
Pages (from-to)	4063-4067
Number of pages	5
Journal	Analytical Chemistry
Volume	66
Issue number	22
DOIs	https://doi.org/10.1021/ac00094a031
State	Published - Nov 1 1994

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10.1021/ac00094a031

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title = "Separation of Fragments up to 570 Bases in Length by Use of 6\% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis",

abstract = "Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.",

author = "Norine Best and Edgar Arriaga and \{Da Chen\}, Yong and Dovichi, \{Norman J.\}",

year = "1994",

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journal = "Analytical Chemistry",

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T1 - Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis

AU - Best, Norine

AU - Arriaga, Edgar

AU - Da Chen, Yong

AU - Dovichi, Norman J.

PY - 1994/11/1

Y1 - 1994/11/1

N2 - Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.

AB - Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.

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UR - https://www.scopus.com/pages/publications/0028536034#tab=citedBy

U2 - 10.1021/ac00094a031

DO - 10.1021/ac00094a031

M3 - Article

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AN - SCOPUS:0028536034

SN - 0003-2700

VL - 66

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EP - 4067

JO - Analytical Chemistry

JF - Analytical Chemistry

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ER -

Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis

Abstract

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