Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis

Norine Best, Edgar Arriaga, Yong Da Chen, Norman J. Dovichi

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.

Original languageEnglish (US)
Pages (from-to)4063-4067
Number of pages5
JournalAnalytical chemistry
Volume66
Issue number22
DOIs
StatePublished - Nov 1 1994

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