Separation of doxorubicin and doxorubicinol by cyclodextrin-modified micellar electrokinetic capillary chromatography

Angela R. Eder, Jennifer S. Chen, Edgar A. Arriaga

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Doxorubicinol (DOXol) is a human metabolite of the chemotherapy agent doxorubicin (DOX), and is associated with dose-dependent cardiotoxicity and decreased drug efficacy. Due to the structural similarities and equal molecular charges of DOXol and DOX, their electrophoretic separation is commonly ineffective. A method for separating and detecting DOX and DOXol, as well as two DOXol enantiomers, was established using cyclodextrin-modified micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. Differential DOXol production was detected in a DOX-sensitive and resistant pair of cell lines, with a 0.08±0.01 fmol limit of detection.

Original languageEnglish (US)
Pages (from-to)3263-3270
Number of pages8
JournalELECTROPHORESIS
Volume27
Issue number16
DOIs
StatePublished - Aug 2006

Keywords

  • Cyclodextrin
  • Doxorubicin
  • Doxorubicinol
  • Laser-induced fluorescence
  • MEKC

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