Abstract
The isocratic separation of six naturally occurring 5′-ribonucleoside monophosphates by reverse-phase high-performance liquid chromatography has been achieved using solvents containing tetramethylammonium, tetraethylammonium, or tetrabutylammonium ions. Their effectiveness in resolving the nucleoside monophosphates increases with increasing akyl chain length (butyl > ethyl > methyl). In an acidic aqueous solvent containing methanol, these ion-pair reagents effect discrete separations of all the nucleoside monophosphates. The systems described also provide for virtually quantitative recovery of the separated nucleotides and utilize phosphate-free solvents comprised of volatile components easily removed by evaporation.
Original language | English (US) |
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Pages (from-to) | 240-245 |
Number of pages | 6 |
Journal | Analytical Biochemistry |
Volume | 107 |
Issue number | 1 |
DOIs | |
State | Published - 1980 |
Bibliographical note
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