Separation of 5′-ribonucleoside monophosphates by ion-pair reverse-phase high-performance liquid chromatography

Timothy F. Walseth, Gustav Graff, Malcolm C. Moos, Nelson D. Goldberg

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The isocratic separation of six naturally occurring 5′-ribonucleoside monophosphates by reverse-phase high-performance liquid chromatography has been achieved using solvents containing tetramethylammonium, tetraethylammonium, or tetrabutylammonium ions. Their effectiveness in resolving the nucleoside monophosphates increases with increasing akyl chain length (butyl > ethyl > methyl). In an acidic aqueous solvent containing methanol, these ion-pair reagents effect discrete separations of all the nucleoside monophosphates. The systems described also provide for virtually quantitative recovery of the separated nucleotides and utilize phosphate-free solvents comprised of volatile components easily removed by evaporation.

Original languageEnglish (US)
Pages (from-to)240-245
Number of pages6
JournalAnalytical Biochemistry
Volume107
Issue number1
DOIs
StatePublished - 1980

Bibliographical note

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Copyright 2017 Elsevier B.V., All rights reserved.

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