Separation and identification of retinoic acid photo-isomers

M. G. Motto, K. L. Facchine, P. F. Hamburg, D. J. Burinsky, R. Dunphy, A. R. Oyler, M. L. Cotter

Research output: Contribution to journalArticlepeer-review

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Retinoic acid was isomerized in ethanol-water (90:10) with fluorescent light. Reversed-phase high-performance liquid chromatography (HPLC) on a 3-μm ODS-2 column with a highly specific mobile phase allowed simultaneous determination of ten retinoic acid isomers that were produced during the photoisomerization. Nine of the isomers were isolated by HPLC and characterized by spectroscopic methods (1H NMR, mass spectrometry and UV). The variation of product distribution with time was determined over the course of the reaction (21 h).

Original languageEnglish (US)
Pages (from-to)255-262
Number of pages8
JournalJournal of Chromatography A
Issue numberC
StatePublished - 1989


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