Retinoic acid was isomerized in ethanol-water (90:10) with fluorescent light. Reversed-phase high-performance liquid chromatography (HPLC) on a 3-μm ODS-2 column with a highly specific mobile phase allowed simultaneous determination of ten retinoic acid isomers that were produced during the photoisomerization. Nine of the isomers were isolated by HPLC and characterized by spectroscopic methods (1H NMR, mass spectrometry and UV). The variation of product distribution with time was determined over the course of the reaction (21 h).
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Chromatography A|
|State||Published - 1989|