Sensitivity Enhancement of HCACO by Using an HMQC Magnetization Transfer Scheme

Youlin Xia, Xiangming Kong, David K. Smith, Yu Liu, David Man, Guang Zhu

Research output: Contribution to journalEditorialpeer-review

10 Scopus citations


Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of 1 H α - 13 C α (R MQ ) is, on average, 1.3 ± 0.4 or 1.7 ± 0.6 times slower than the single-quantum relaxation rate of 13 C α (R C ) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has been developed. We demonstrate that this new experiment is 23 and 55% more sensitive than the original HSQC version of the HCACO experiment, at constant times of 7 and 27 ms, respectively, for a sample of the BC domain of the ciliary neurotrophic factor receptor protein dissolved in D 2 O at 20°C.

Original languageEnglish (US)
Pages (from-to)407-410
Number of pages4
JournalJournal of Magnetic Resonance
Issue number2
StatePublished - Apr 2000
Externally publishedYes

Bibliographical note

Funding Information:
This work is supported by grants (HKUST6197/97M and HKUST6038/ 98M) from the Research Grant Council of Hong Kong. We thank Dr. K. H. Sze for critical discussions. The Hong Kong Biotechnology Research Institute is acknowledged for the purchase of the 500-MHz NMR spectrometer.


  • C-labeled proteins
  • HMQC
  • NMR


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