Semi-constitutive expression of an Arabidopsis thaliana α-tubulin gene

Jeffrey L. Carpenter, Steven D. Kopczak, D. Peter Snustad, Carolyn D. Silflow

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

In Arabidopsis tissues, the pool of tubulin protein is provided by the expression of multiple α-tubulin and β-tubulin genes. Previous evidence suggested that the TUA2 α-tubulin gene was expressed in all organs of mature plants. We now report a more detailed analysis of TUA2 expression during plant development. Chimeric genes containing TUA2 5′-flanking DNA fused to the β-glucuronidase (GUS) coding region were used to create transgenic Arabidopsis plants. Second-generation progeny of regenerated plants were analyzed by histochemical assay to localize GUS expression. GUS activity was seen throughout plant development and in nearly all tissues. The blue product of GUS activity accumulated to the highest levels in tissues with actively dividing and elongating cells. GUS activity was not detected in a few plant tissues, suggesting that, though widely expressed, the TUA2 promoter is not constitutively active.

Original languageEnglish (US)
Pages (from-to)937-942
Number of pages6
JournalPlant molecular biology
Volume21
Issue number5
DOIs
StatePublished - Mar 1 1993

Keywords

  • Arabidopsis
  • gene expression
  • transformation
  • α-tubulin
  • β-glucuronidase

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