Semaphorin 4A is dynamically regulated during thymocyte development in mice

Grace E. Linder, Pavlina D. Chuntova, Bryce T. McLelland, Leonor Añó, Udochukwu C. Obodo, Nathaniel J. Crider, David J. Matthes, Marcos E. García-Ojeda, Jennifer O. Manilay, Devavani Chatterjea

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Semaphorins are important regulators of peripheral T and B-cell mediated immune responses in mice and humans. Modulatory roles of semaphorins in T cell development are also being characterized. We carefully analyzed the gene expression and protein levels of semaphorins 4A, 4D, and 7A at various developmental stages of T cell maturation in the thymus of C57BL/6 mice. Sema7a was expressed at very low levels, while Sema4d was abundant at all developmental stages of mouse thymocytes. We found the most interesting pattern of gene regulation and protein localization for semaphorin 4A. Both semaphorin 4A mRNA and protein were clearly detected on the earliest progenitors and were downregulated through thymic development. SEMA4A protein also showed a distinct cortico-medullary pattern of localization. Our findings contribute to an understanding of the complex roles played by semaphorins in the network of spatially and temporally regulated cues underpinning T cell development in the thymus.

Original languageEnglish (US)
Pages (from-to)150-158
Number of pages9
JournalCellular Immunology
Volume281
Issue number2
DOIs
StatePublished - Feb 1 2013

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Semaphorins
Thymocytes
T-Lymphocytes
Thymus Gland
Proteins
Inbred C57BL Mouse
Cues
B-Lymphocytes
Down-Regulation
Gene Expression
Messenger RNA

Keywords

  • Development
  • Murine
  • Regulation
  • Semaphorin 4A
  • Thymocytes

Cite this

Linder, G. E., Chuntova, P. D., McLelland, B. T., Añó, L., Obodo, U. C., Crider, N. J., ... Chatterjea, D. (2013). Semaphorin 4A is dynamically regulated during thymocyte development in mice. Cellular Immunology, 281(2), 150-158. https://doi.org/10.1016/j.cellimm.2013.03.006

Semaphorin 4A is dynamically regulated during thymocyte development in mice. / Linder, Grace E.; Chuntova, Pavlina D.; McLelland, Bryce T.; Añó, Leonor; Obodo, Udochukwu C.; Crider, Nathaniel J.; Matthes, David J.; García-Ojeda, Marcos E.; Manilay, Jennifer O.; Chatterjea, Devavani.

In: Cellular Immunology, Vol. 281, No. 2, 01.02.2013, p. 150-158.

Research output: Contribution to journalArticle

Linder, GE, Chuntova, PD, McLelland, BT, Añó, L, Obodo, UC, Crider, NJ, Matthes, DJ, García-Ojeda, ME, Manilay, JO & Chatterjea, D 2013, 'Semaphorin 4A is dynamically regulated during thymocyte development in mice', Cellular Immunology, vol. 281, no. 2, pp. 150-158. https://doi.org/10.1016/j.cellimm.2013.03.006
Linder GE, Chuntova PD, McLelland BT, Añó L, Obodo UC, Crider NJ et al. Semaphorin 4A is dynamically regulated during thymocyte development in mice. Cellular Immunology. 2013 Feb 1;281(2):150-158. https://doi.org/10.1016/j.cellimm.2013.03.006
Linder, Grace E. ; Chuntova, Pavlina D. ; McLelland, Bryce T. ; Añó, Leonor ; Obodo, Udochukwu C. ; Crider, Nathaniel J. ; Matthes, David J. ; García-Ojeda, Marcos E. ; Manilay, Jennifer O. ; Chatterjea, Devavani. / Semaphorin 4A is dynamically regulated during thymocyte development in mice. In: Cellular Immunology. 2013 ; Vol. 281, No. 2. pp. 150-158.
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