Selenium reduces enterohemorrhagic Escherichia coli O157:H7 verotoxin production and globotriaosylceramide receptor expression on host cells

Meera Surendran-Nair, Anup Kollanoor-Johny, Sangeetha Ananda-Baskaran, Carol Norris, Ji Young Lee, Kumar Venkitanarayanan

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Aim: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. Materials & methods: The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Results & conclusions: Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.

Original languageEnglish (US)
Pages (from-to)745-756
Number of pages12
JournalFuture Microbiology
Volume11
Issue number6
DOIs
StatePublished - Jun 2016

Keywords

  • E. coli O157:H7
  • Gb3 receptor
  • selenium
  • verotoxin

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