TY - JOUR
T1 - Selective Loading and Variations in the miRNA Profile of Extracellular Vesicles from Endothelial-like Cells Cultivated under Normoxia and Hypoxia
AU - Robert, Anny Waloski
AU - Marcon, Bruna Hilzendeger
AU - Angulski, Addeli Bez Batti
AU - Martins, Sharon de Toledo
AU - Leitolis, Amanda
AU - Stimamiglio, Marco Augusto
AU - Senegaglia, Alexandra Cristina
AU - Correa, Alejandro
AU - Alves, Lysangela Ronalte
N1 - Funding Information:
This research was supported by MCTI/CNPq/MS (Grant number 404656/2012-9). A.C. and received financial support from Fundação Araucária (Grant number 1005/2013).
Publisher Copyright:
© 2022 by the authors.
PY - 2022/9
Y1 - 2022/9
N2 - Endothelial-like cells may be obtained from CD133+ mononuclear cells isolated from human umbilical cord blood (hUCB) and expanded using endothelial-inducing medium (E-CD133 cells). Their use in regenerative medicine has been explored by the potential not only to form vessels but also by the secretion of bioactive elements. Extracellular vesicles (EVs) are prominent messengers of this paracrine activity, transporting bioactive molecules that may guide cellular response under different conditions. Using RNA-Seq, we characterized the miRNA content of EVs derived from E-CD133 cells cultivated under normoxia (N-EVs) and hypoxia (H-EVs) and observed that changing the O2 status led to variations in the selective loading of miRNAs in the EVs. In silico analysis showed that among the targets of differentially loaded miRNAs, there are transcripts involved in pathways related to cell growth and survival, such as FoxO and HIF-1 pathways. The data obtained reinforce the pro-regenerative potential of EVs obtained from E-CD133 cells and shows that fine tuning of their properties may be regulated by culture conditions.
AB - Endothelial-like cells may be obtained from CD133+ mononuclear cells isolated from human umbilical cord blood (hUCB) and expanded using endothelial-inducing medium (E-CD133 cells). Their use in regenerative medicine has been explored by the potential not only to form vessels but also by the secretion of bioactive elements. Extracellular vesicles (EVs) are prominent messengers of this paracrine activity, transporting bioactive molecules that may guide cellular response under different conditions. Using RNA-Seq, we characterized the miRNA content of EVs derived from E-CD133 cells cultivated under normoxia (N-EVs) and hypoxia (H-EVs) and observed that changing the O2 status led to variations in the selective loading of miRNAs in the EVs. In silico analysis showed that among the targets of differentially loaded miRNAs, there are transcripts involved in pathways related to cell growth and survival, such as FoxO and HIF-1 pathways. The data obtained reinforce the pro-regenerative potential of EVs obtained from E-CD133 cells and shows that fine tuning of their properties may be regulated by culture conditions.
KW - CD133 cells
KW - endothelial-like cells
KW - extracellular vesicles
KW - hypoxia
KW - miRNA
UR - http://www.scopus.com/inward/record.url?scp=85138599307&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85138599307&partnerID=8YFLogxK
U2 - 10.3390/ijms231710066
DO - 10.3390/ijms231710066
M3 - Article
C2 - 36077462
AN - SCOPUS:85138599307
SN - 1661-6596
VL - 23
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 17
M1 - 10066
ER -