Selective in vitro photothermal nano-therapy of MRSA infections mediated by IgG conjugated gold nanoparticles

Lucian Mocan, Cristian Matea, Flaviu A. Tabaran, Ofelia Mosteanu, Teodora Pop, Cosmin Puia, Lucia Agoston-Coldea, Diana Gonciar, Erszebet Kalman, Gabriela Zaharie, Cornel Iancu, Teodora Mocan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

There are serious systemic infections associated with methicillin-resistant Staphylococcus aureus (MRSA) and several other types of bacteria leading to the deaths of millions of people globally. This type of mortality is generally caused by the increasing number of antibiotic-resistant organisms, a consequence of evolution via natural selection. After the synthesis of gold nanoparticles (GNPs) by wet chemistry, bio-functionalization with IgG molecules was performed. Following administration of IgG-GNPs to MRSA cultures at various concentrations and various incubation time laser irradiation was performed. To assess the selectivity and specificity of the proposed treatment the following methods were used: flow cytometry, contrast phase microscopy, and by fluorescence microscopy. The results in our study indicate that following administration of IgG-GNPs biomolecule an extended and selective bacterial death occurs following laser irradiation in a dose dependent manner. Therefore, the new findings might impel studies on these antibacterial nanomaterials and their biological and medical applications.

Original languageEnglish (US)
Article number39466
JournalScientific Reports
Volume6
DOIs
StatePublished - Dec 23 2016
Externally publishedYes

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Methicillin-Resistant Staphylococcus aureus
Gold
Nanoparticles
Immunoglobulin G
Lasers
Infection
Phase-Contrast Microscopy
Nanostructures
Genetic Selection
Fluorescence Microscopy
Flow Cytometry
Therapeutics
Anti-Bacterial Agents
Bacteria
Mortality
In Vitro Techniques

Cite this

Mocan, L., Matea, C., Tabaran, F. A., Mosteanu, O., Pop, T., Puia, C., ... Mocan, T. (2016). Selective in vitro photothermal nano-therapy of MRSA infections mediated by IgG conjugated gold nanoparticles. Scientific Reports, 6, [39466]. https://doi.org/10.1038/srep39466

Selective in vitro photothermal nano-therapy of MRSA infections mediated by IgG conjugated gold nanoparticles. / Mocan, Lucian; Matea, Cristian; Tabaran, Flaviu A.; Mosteanu, Ofelia; Pop, Teodora; Puia, Cosmin; Agoston-Coldea, Lucia; Gonciar, Diana; Kalman, Erszebet; Zaharie, Gabriela; Iancu, Cornel; Mocan, Teodora.

In: Scientific Reports, Vol. 6, 39466, 23.12.2016.

Research output: Contribution to journalArticle

Mocan, L, Matea, C, Tabaran, FA, Mosteanu, O, Pop, T, Puia, C, Agoston-Coldea, L, Gonciar, D, Kalman, E, Zaharie, G, Iancu, C & Mocan, T 2016, 'Selective in vitro photothermal nano-therapy of MRSA infections mediated by IgG conjugated gold nanoparticles', Scientific Reports, vol. 6, 39466. https://doi.org/10.1038/srep39466
Mocan, Lucian ; Matea, Cristian ; Tabaran, Flaviu A. ; Mosteanu, Ofelia ; Pop, Teodora ; Puia, Cosmin ; Agoston-Coldea, Lucia ; Gonciar, Diana ; Kalman, Erszebet ; Zaharie, Gabriela ; Iancu, Cornel ; Mocan, Teodora. / Selective in vitro photothermal nano-therapy of MRSA infections mediated by IgG conjugated gold nanoparticles. In: Scientific Reports. 2016 ; Vol. 6.
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