Selective characterization of proteins on nanoscale concave surfaces

Xi Qian, Utthara Rameshbabu, Jonathan S. Dordick, Richard W. Siegel

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Nanoscale curvature plays a critical role in nanostructure-biomolecule interactions, yet the understanding of such effects in concave nanostructures is still very limited. Because concave nanostructures usually possess convex surface curvatures as well, it is challenging to selectively study the proteins on concave surfaces alone. In this work, we have developed a novel and facile method to address this issue by desorbing proteins on the external surfaces of hollow gold nanocages (AuNG), allowing the selective characterization of retained proteins immobilized on their internal concave surfaces. The selective desorption of proteins was achieved via varying the solution ionic strength, and was demonstrated by both calculation and experimental comparison with non-hollow nanoparticles. This method has created a new platform for the discrete observation of proteins adsorbed inside AuNG hollow cores, and this work suggests an expanded biomedical application space for hollow nanomaterials.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
StatePublished - Jan 2016

Bibliographical note

Funding Information:
This work was supported by the Nanoscale Science and Engineering Initiative of the National Science Foundation under Grant DMR-0642573 .

Publisher Copyright:
© 2015 Elsevier Ltd.


  • Concave nanostructure
  • Gold nanoparticle
  • Nano-bio interaction
  • Protein adsorption


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