Selection for seed size has uneven effects on specialized metabolite abundance in oat (Avena sativa L.)

Lauren J. Brzozowski, Haixiao Hu, Malachy T. Campbell, Corey D. Broeckling, Melanie Caffe, Lucıa Gutierrez, Kevin P. Smith, Mark E. Sorrells, Michael A. Gore, Jean Luc Jannink

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Plant breeding strategies to optimize metabolite profiles are necessary to develop health-promoting food crops. In oats (Avena sativa L.), seed metabolites are of interest for their antioxidant properties, yet have not been a direct target of selection in breeding. In a diverse oat germplasm panel spanning a century of breeding, we investigated the degree of variation of these specialized metabolites and how it has been molded by selection for other traits, like yield components. We also ask if these patterns of variation persist in modern breeding pools. Integrating genomic, transcriptomic, metabolomic, and phenotypic analyses for three types of seed specialized metabolites-avenanthramides, avenacins, and avenacosides-we found reduced heritable genetic variation in modern germplasm compared with diverse germplasm, in part due to increased seed size associated with more intensive breeding. Specifically, we found that abundance of avenanthramides increases with seed size, but additional variation is attributable to expression of biosynthetic enzymes. In contrast, avenacoside abundance decreases with seed size and plant breeding intensity. In addition, these different specialized metabolites do not share large-effect loci. Overall, we show that increased seed size associated with intensive plant breeding has uneven effects on the oat seed metabolome, but variation also exists independently of seed size to use in plant breeding. This work broadly contributes to our understanding of how plant breeding has influenced plant traits and tradeoffs between traits (like growth and defense) and the genetic bases of these shifts.

Original languageEnglish (US)
Article numberjkab419
JournalG3: Genes, Genomes, Genetics
Volume12
Issue number3
DOIs
StatePublished - Mar 2022

Bibliographical note

Funding Information:
Funding for this research was provided by United States Department of Agriculture - National Institute of Food and Agriculture - Agriculture and Food Research Initiative (USDA-NIFA-AFRI) 2017-67007-26502 and Hatch Project 149-577.

Funding Information:
J.L.J., M.A.G., and M.E.S. designed the research. L.J.B., H.H., and M.T.C. analyzed the data, and H.H., M.T.C., M.C., L.G., K.P.S., and M.E.S. conducted experiments. C.D.B. conducted the metabolomics extraction, measurement, and data processing. L.J.B., M.A.G., and J.L.J. wrote the manuscript and all coauthors were involved in editing the manuscript. Funding for this research was provided by United States Department of Agriculture - National Institute of Food and Agriculture - Agriculture and Food Research Initiative (USDANIFA-AFRI) 2017-67007-26502 and Hatch Project 149-577.

Publisher Copyright:
© The Author(s) 2021

Keywords

  • Avena sativa
  • avenacin
  • avenacoside
  • avenanthramide
  • eQTL
  • plant breeding
  • seed size
  • specialized metabolism
  • transcriptomics

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.

Fingerprint

Dive into the research topics of 'Selection for seed size has uneven effects on specialized metabolite abundance in oat (Avena sativa L.)'. Together they form a unique fingerprint.

Cite this