SECRET AGENT, an Arabidopsis thaliana O-GlcNAc transferase, modifies the Plum pox virus capsid protein

Cheryl L. Scott; Lynn M. Hartweck; José de Jesús Pérez; Dinghu Chen; Juan Antonio García; Neil E. Olszewski

doi:10.1016/j.febslet.2006.09.046

SECRET AGENT, an Arabidopsis thaliana O-GlcNAc transferase, modifies the Plum pox virus capsid protein

Cheryl L. Scott, Lynn M. Hartweck, José de Jesús Pérez, Dinghu Chen, Juan Antonio García, Neil E. Olszewski

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The capsid protein of Plum pox virus (PPV-CP) is modified with O-linked GlcNAc (O-GlcNAc). While Arabidopsis has two O-GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV-CP and that the modification plays a role in the infection process. Here, we show that when co-expressed in Escherichia coli SEC modifies PPV-CP. Deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coli.

Original language	English (US)
Pages (from-to)	5829-5835
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	25
DOIs	https://doi.org/10.1016/j.febslet.2006.09.046
State	Published - Oct 30 2006

Bibliographical note

Funding Information:
This work was supported by Grants from the National Science Foundation (MCB-0112826 and -0516690) to NEO and by the US Department of Energy (DE-FG02-02ER15353) to NEO and LMH and grants BIO2004-02687 from Spanish MEC and SP22-CT-2004 from European Union to J.A.G.

Keywords

Arabidopsis thaliana
Capsid protein
O-GlcNAc
O-GlcNAc transferase
Plum pox virus
SECRET AGENT

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title = "SECRET AGENT, an Arabidopsis thaliana O-GlcNAc transferase, modifies the Plum pox virus capsid protein",

abstract = "The capsid protein of Plum pox virus (PPV-CP) is modified with O-linked GlcNAc (O-GlcNAc). While Arabidopsis has two O-GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV-CP and that the modification plays a role in the infection process. Here, we show that when co-expressed in Escherichia coli SEC modifies PPV-CP. Deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coli.",

keywords = "Arabidopsis thaliana, Capsid protein, O-GlcNAc, O-GlcNAc transferase, Plum pox virus, SECRET AGENT",

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note = "Funding Information: This work was supported by Grants from the National Science Foundation (MCB-0112826 and -0516690) to NEO and by the US Department of Energy (DE-FG02-02ER15353) to NEO and LMH and grants BIO2004-02687 from Spanish MEC and SP22-CT-2004 from European Union to J.A.G. ",

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doi = "10.1016/j.febslet.2006.09.046",

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AU - de Jesús Pérez, José

AU - Chen, Dinghu

AU - García, Juan Antonio

AU - Olszewski, Neil E.

N1 - Funding Information: This work was supported by Grants from the National Science Foundation (MCB-0112826 and -0516690) to NEO and by the US Department of Energy (DE-FG02-02ER15353) to NEO and LMH and grants BIO2004-02687 from Spanish MEC and SP22-CT-2004 from European Union to J.A.G.

PY - 2006/10/30

Y1 - 2006/10/30

N2 - The capsid protein of Plum pox virus (PPV-CP) is modified with O-linked GlcNAc (O-GlcNAc). While Arabidopsis has two O-GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV-CP and that the modification plays a role in the infection process. Here, we show that when co-expressed in Escherichia coli SEC modifies PPV-CP. Deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coli.

AB - The capsid protein of Plum pox virus (PPV-CP) is modified with O-linked GlcNAc (O-GlcNAc). While Arabidopsis has two O-GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV-CP and that the modification plays a role in the infection process. Here, we show that when co-expressed in Escherichia coli SEC modifies PPV-CP. Deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coli.

KW - Arabidopsis thaliana

KW - Capsid protein

KW - O-GlcNAc

KW - O-GlcNAc transferase

KW - Plum pox virus

KW - SECRET AGENT

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SECRET AGENT, an Arabidopsis thaliana O-GlcNAc transferase, modifies the Plum pox virus capsid protein

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