Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

Sang Hyun Oh; Amarendra K. Singh; Paul H. Bessette; Sophia A. Kenrick; Jeffrey J. Rice; Jiangrong Qian; Patrick S. Daugherty; H. T. Soh

Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

Sang Hyun Oh
, Amarendra K. Singh
, Paul H. Bessette
, Sophia A. Kenrick
, Jeffrey J. Rice
, Jiangrong Qian
, Patrick S. Daugherty
, H. T. Soh

Electrical and Computer Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Scopus citations

Abstract

A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.

Original language	English (US)
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference
Subtitle of host publication	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Japan Academic Association Inc
Pages	975-977
Number of pages	3
State	Published - Jan 1 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

Keywords

High-throughput screening
In vitro selection
Magnetic cell sorting

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Oh, S. H., Singh, A. K., Bessette, P. H., Kenrick, S. A., Rice, J. J., Qian, J., Daugherty, P. S., & Soh, H. T. (2006). Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 975-977). Japan Academic Association Inc.

Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter. / Oh, Sang Hyun; Singh, Amarendra K.; Bessette, Paul H. et al.
Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 975-977.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Oh, SH, Singh, AK, Bessette, PH, Kenrick, SA, Rice, JJ, Qian, J, Daugherty, PS & Soh, HT 2006, Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 975-977, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06.

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title = "Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter",

abstract = "A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.",

keywords = "High-throughput screening, In vitro selection, Magnetic cell sorting",

author = "Oh, \{Sang Hyun\} and Singh, \{Amarendra K.\} and Bessette, \{Paul H.\} and Kenrick, \{Sophia A.\} and Rice, \{Jeffrey J.\} and Jiangrong Qian and Daugherty, \{Patrick S.\} and Soh, \{H. T.\}",

year = "2006",

month = jan,

day = "1",

language = "English (US)",

pages = "975--977",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference",

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note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - GEN

T1 - Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

AU - Oh, Sang Hyun

AU - Singh, Amarendra K.

AU - Bessette, Paul H.

AU - Kenrick, Sophia A.

AU - Rice, Jeffrey J.

AU - Qian, Jiangrong

AU - Daugherty, Patrick S.

AU - Soh, H. T.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.

AB - A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.

KW - High-throughput screening

KW - In vitro selection

KW - Magnetic cell sorting

UR - https://www.scopus.com/pages/publications/84902491323

UR - https://www.scopus.com/pages/publications/84902491323#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:84902491323

SP - 975

EP - 977

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference

PB - Japan Academic Association Inc

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

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