Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

Sang Hyun Oh, Amarendra K. Singh, Paul H. Bessette, Sophia A. Kenrick, Jeffrey J. Rice, Jiangrong Qian, Patrick S. Daugherty, H. T. Soh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference
Subtitle of host publication10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages975-977
Number of pages3
StatePublished - Jan 1 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Keywords

  • High-throughput screening
  • In vitro selection
  • Magnetic cell sorting

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