Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

Sang Hyun Oh; Amarendra K. Singh; Paul H. Bessette; Sophia A. Kenrick; Jeffrey J. Rice; Jiangrong Qian; Patrick S. Daugherty; H. T. Soh

Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

Sang Hyun Oh, Amarendra K. Singh, Paul H. Bessette, Sophia A. Kenrick, Jeffrey J. Rice, Jiangrong Qian, Patrick S. Daugherty, H. T. Soh

Electrical and Computer Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.

Original language	English (US)
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference
Subtitle of host publication	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Japan Academic Association Inc
Pages	975-977
Number of pages	3
State	Published - Jan 1 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

Keywords

High-throughput screening
In vitro selection
Magnetic cell sorting

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Oh, S. H., Singh, A. K., Bessette, P. H., Kenrick, S. A., Rice, J. J., Qian, J., Daugherty, P. S., & Soh, H. T. (2006). Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 975-977). Japan Academic Association Inc.

Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter. / Oh, Sang Hyun; Singh, Amarendra K.; Bessette, Paul H. et al.
Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 975-977.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Oh, SH, Singh, AK, Bessette, PH, Kenrick, SA, Rice, JJ, Qian, J, Daugherty, PS & Soh, HT 2006, Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 975-977, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06.

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title = "Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter",

abstract = "A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.",

keywords = "High-throughput screening, In vitro selection, Magnetic cell sorting",

author = "Oh, {Sang Hyun} and Singh, {Amarendra K.} and Bessette, {Paul H.} and Kenrick, {Sophia A.} and Rice, {Jeffrey J.} and Jiangrong Qian and Daugherty, {Patrick S.} and Soh, {H. T.}",

year = "2006",

month = jan,

day = "1",

language = "English (US)",

pages = "975--977",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference",

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note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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T1 - Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

AU - Oh, Sang Hyun

AU - Singh, Amarendra K.

AU - Bessette, Paul H.

AU - Kenrick, Sophia A.

AU - Rice, Jeffrey J.

AU - Qian, Jiangrong

AU - Daugherty, Patrick S.

AU - Soh, H. T.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.

AB - A micromachined, two-stage, continuous-flow, micro-magnetic cell sorter is presented. Ferromagnetic microstrips were patterned in the micro-channel to generate a large magnetic field gradient, and the resulting magnetophoretic force deflected magnetically labeled cells toward a desired outlet in a laminar-flow buffer switching system. Using the sorter and superparamagnetic microbeads, we have demonstrated screening and enrichment of a peptide library based on its affinity towards the target antibody.

KW - High-throughput screening

KW - In vitro selection

KW - Magnetic cell sorting

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UR - http://www.scopus.com/inward/citedby.url?scp=84902491323&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84902491323

SP - 975

EP - 977

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference

PB - Japan Academic Association Inc

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Screening of molecular libraries using the continuous-flow, micro-magnetic cell sorter

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