Screening Common Bean Genotypes for Tolerance to Low Zinc Availability Using a Chelate-Buffered Hydroponics System

J. D. Knight, D. Gangotena, D. L. Allan, C. J. Rosen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A chelate-buffered hydroponics system was assessed for its ability to induce zinc (Zn) deficiency in common bean and for its usefulness as a tool to select genotypes tolerant of Zn deficiency stress. Twenty-two common bean genotypes were evaluated for tolerance to Zn deficient conditions using the buffered hydroponics system. Relative yield (comparing growth under low Zn to adequate Zn conditions) and Zn and phosphorus (P) accumulation in plants were measured. Significant Zn deficiency stress was induced in all of the genotypes with relative yields ranging from 11.5 to 52.6%. Six genotypes were identified as being tolerant to low Zn. These six genotypes (E 101, AND 684, LSA 102, SUG 55, PVA 773, and CENTRO) all had relative yields >29%, were able to accumulate >60 μg Zn per plant from solution and were better able to regulate P uptake to avoid excessive P accumulation than the other genotypes. In a second experiment, a subset of seven genotypes grown in the chelate-buffered hydroponics systems were compared directly to plants grown in field soil. The two systems correlated well for total dry weight (r = 0.57, P < 0.001), shoot Zn content (μg g-1) (r = 0.58, P < 0.001), and total Zn content (μg g-1) (r = 0.64, < 0.001) when plants were grown under low Zn conditions.

Original languageEnglish (US)
Pages (from-to)275-293
Number of pages19
JournalJournal of Plant Nutrition
Volume27
Issue number2
DOIs
StatePublished - 2004

Bibliographical note

Funding Information:
This research was financed by a US-AID Bean/Cowpea Project awarded to the Instituto Nacional de Investigaciones Agropecuarios (INIAP) Estacion Experimental in Santa Catalina, Quito, Ecuador and the University of Minnesota. Thanks to Dr. Yanping Liu for technical support. Seed from GIZA 6 was obtained from Dr. P. Graham at the University of Minnesota. Seed from the remaining 21 genotypes was obtained from INIAP.

Keywords

  • Bean genotypes
  • Chelate-buffered
  • Phosphorus
  • Relative yield
  • Soil screening
  • Zinc deficiency

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