Scalable, semi-automated fluorescence reduction neutralization assay for qualitative assessment of Ebola virus-neutralizing antibodies in human clinical samples

Elena N. Postnikova, James Pettitt, Collin J. Van Ryn, Michael R. Holbrook, Laura Bollinger, Shuīqìng Yú, Yíngyún Caì, Janie Liang, Michael C. Sneller, Peter B. Jahrling, Lisa E. Hensley, Jens H. Kuhn, Mosoka P. Fallah, Richard S. Bennett, Cavan Reilly

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Antibody titers against a viral pathogen are typically measured using an antigen binding assay, such as an enzyme-linked immunosorbent assay (ELISA), which only measures the ability of antibodies to identify a viral antigen of interest. Neutralization assays measure the presence of virus-neutralizing antibodies in a sample. Traditional neutralization assays, such as the plaque reduction neutralization test (PRNT), are often difficult to use on a large scale due to being both labor and resource intensive. Here we describe an Ebola virus fluorescence reduction neutralization assay (FRNA), which tests for neutralizing antibodies, that requires only a small volume of sample in a 96-well format and is easy to automate. The readout of the FRNA is the percentage of Ebola virus-infected cells measured with an optical reader or overall chemiluminescence that can be generated by multiple reading platforms. Using blinded human clinical samples (EVD survivors or contacts) obtained in Liberia during the 2013–2016 Ebola virus disease outbreak, we demonstrate there was a high degree of agreement between the FRNA-measured antibody titers and the Filovirus Animal Non-clinical Group (FANG) ELISA titers with the FRNA providing information on the neutralizing capabilities of the antibodies.

Original languageEnglish (US)
Article numbere0221407
JournalPloS one
Volume14
Issue number8
DOIs
StatePublished - Aug 1 2019

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

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    Postnikova, E. N., Pettitt, J., Van Ryn, C. J., Holbrook, M. R., Bollinger, L., Yú, S., Caì, Y., Liang, J., Sneller, M. C., Jahrling, P. B., Hensley, L. E., Kuhn, J. H., Fallah, M. P., Bennett, R. S., & Reilly, C. (2019). Scalable, semi-automated fluorescence reduction neutralization assay for qualitative assessment of Ebola virus-neutralizing antibodies in human clinical samples. PloS one, 14(8), [e0221407]. https://doi.org/10.1371/journal.pone.0221407