Sargachromanol G regulates the expression of osteoclastogenic factors in human osteoblast-like MG-63 cells

Weon Jong Yoon, Soo Jin Heo, Sang Chul Han, Hye Ja Lee, Gyeoung Jin Kang, Eun Jin Yang, Sun Soon Park, Hee Kyoung Kang, Eun Sook Yoo

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Bone diseases are characterized by the presence of pro-inflammatory cytokines that regulate bone turnover. The receptor activator of NF-κB ligand (RANKL) is a soluble osteoblast-derived protein that induces bone resorption through osteoclast differentiation and activation. Sargachromanol G (SG) was isolated from the brown algae Sargassum siliquastrum; SG has anti-osteoclastogenic activity, but its mechanism of action and its active components remain largely unknown. In the present study, we investigated the anti-osteoclastogenic effects of SG on the expression of interleukin-1β (IL-1β)-induced osteoclastogenic factors (PGE2, COX-2, IL-6, OPG, and RANKL) in the human osteoblast cell line MG-63. We also examined the role of the nuclear factor-κB (NF-κB) and the mitogen-activated protein kinase (MAPK) signaling pathways in IL-1β-stimulated MG-63 cells. SG dose-dependently inhibited the production of osteoclastogenic factors in MG-63 cells. SG also inhibited phosphorylation of MAPK (ERK1/2, p38, and JNK) and NF-κB (p65, p50, and IκB-α). These results suggest that the anti-osteoporotic effect of SG may be because of the modulation of osteoclastogenic factors via suppression of MAPK and NF-κB activation.

Original languageEnglish (US)
Pages (from-to)3273-3279
Number of pages7
JournalFood and Chemical Toxicology
Volume50
Issue number9
DOIs
StatePublished - Sep 2012

Bibliographical note

Funding Information:
This research was supported by the research programs of the Korea Ocean Research and Development Institute (PE98592).

Keywords

  • MAPK
  • NF-κB
  • Osteoclastogenic factor
  • RANKL
  • Sargachromanol G

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