Salt and stress synergize H. pylori-induced gastric lesions, cell proliferation, and p21 expression in Mongolian gerbils

Armando Gamboa-Dominguez, Tom Ubbelohde, Milena Saqui-Salces, Luis Romano-Mazzoti, Minerva Cervantes, Claudia Domínguez-Fonseca, Maria De La Luz Estreber, Guillermo M. Ruíz-Palacios

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Our aim was to determine if salt and stress enhance Helicobacter pylori (Hp) lesions in Meriones unguiculatus. Two hundred seventy-eight pathogen-free gerbils were allocated to seven groups: Hp-Sydney strain (45), 8% higher-salt diet (38), stress (60% space reduction/water immersion; 36), Hp + salt (33), Hp + stress (34), N-methyl-N-nitro-N-nitrosoguanidine (34), and sham (58). Gerbils were sacrificed at 1 week (67), 12 weeks (73), 52 weeks (65), and 68 weeks (73). Sydney, Padova, and Lauren classifications were blindly used. Proliferation, p53, p21, and apoptosis were assessed. Follicular active gastritis (grade 2/3) was observed in 10% of Hp gerbils, 38% of Hp + salt gerbils, and 29% of Hp + stress gerbils at 52 weeks and 67%, 83%, and 43% at 68 weeks (P < 0.05). Heterotopic proliferative glands were identified in synergy groups from 52 weeks, with increases in their number and size by 68 weeks. Higher proliferative rates were observed in Hp+salt gerbils (P < 0.0001), and p21 overexpression in Hp+salt and Hp+stress gerbils (both P's < 0.0001), by 68 weeks, without p53 increases. We conclude that salt and stress synergize Hp damage and increase pseudo-invasive gland foci.

Original languageEnglish (US)
Pages (from-to)1517-1526
Number of pages10
JournalDigestive Diseases and Sciences
Volume52
Issue number6
DOIs
StatePublished - Jun 2007

Keywords

  • Carcinogenesis
  • Gerbils
  • Helicobacter pylori
  • Salt
  • Stress
  • p21

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    Gamboa-Dominguez, A., Ubbelohde, T., Saqui-Salces, M., Romano-Mazzoti, L., Cervantes, M., Domínguez-Fonseca, C., De La Luz Estreber, M., & Ruíz-Palacios, G. M. (2007). Salt and stress synergize H. pylori-induced gastric lesions, cell proliferation, and p21 expression in Mongolian gerbils. Digestive Diseases and Sciences, 52(6), 1517-1526. https://doi.org/10.1007/s10620-006-9524-3