Ground beef products have been implicated as the vehicle for the transmission of Salmonella in a number of outbreaks. Although carcass surface interventions have proven effective, Salmonella contamination in ground beef still occurs. Recent studies indicate that deep tissue lymph nodes (DTLNs) may be an important source of Salmonella contamination in ground beef products. We developed a stochastic simulation model covering the pre-to post-harvest stages to assess the relative contribution of DTLN as compared with carcass surface, to Salmonella in ground beef, and the impact of various pre- and post-harvest interventions. The model addressed fed and cull cattle, and in high and low prevalence seasons. Contamination from carcass surfaces and DTLNs was simulated separately. Linear relationships were used to describe the changes of Salmonella surface concentration and prevalence at different processing stages. The baseline results indicate that on average over 90% of the Salmonella CFU load in a 2000lb (907kg) production lot originates from DTLN contamination as compared with carcass surface contamination. The relative contribution of DTLN contamination was fairly robust to changes in model parameters for ground beef from fed cattle, while it was comparatively more sensitive to changes in model parameters for cull cattle. The predicted mean Salmonella CFU load from DTLN contamination was considerably greater in ground beef production lots from fed cattle compared with cull cattle. Correspondingly, our scenario analysis suggested that generic pre-harvest interventions which can reduce Salmonella contamination in DTLNs would cause a greater total CFU load reduction in ground beef production lots from fed cattle compared with cull cattle. The study provides some valuable information for prioritizing control measures targeted at Salmonella contamination from the beef carcass surface or DTLNs based on the current knowledge.
Bibliographical noteFunding Information:
This project is funded in part by the The Beef Checkoff and the USDA National Institutes of Food and Agriculture (Award No. 2011-51110-31081 ). The authors are grateful to Drs. Terrance M. Arthur and Joseph M. Bosilevac from USDA/ARS for kindly providing scientific advice and experimental data to define key model parameters, and Drs. Darby Murphy and Sarah E. Strayer from University of Minnesota for contributing to literature search and screening. The author Dr. H. Scott Hurd passed away before the manuscript submission. The rest of the authors acknowledge his significant contributions in designing, conducting, and preparing manuscript of this project.
© 2014 Elsevier Ltd.
- Carcass surface
- Ground beef
- Lymph node
- Risk assessment