Safety and Efficacy of Rose Bengal Derivatives for Glial Scar Ablation in Chronic Spinal Cord Injury

Nandadevi Patil, Vincent Truong, MacKenzie H. Holmberg, Nicolas S. Lavoie, Mark R. McCoy, James R Dutton, Eric G. Holmberg, Ann M Parr

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

There are no effective therapies available currently to ameliorate loss of function for patients with spinal cord injuries (SCIs). In addition, proposed treatments that demonstrated functional recovery in animal models of acute SCI have failed almost invariably when applied to chronic injury models. Glial scar formation in chronic injury is a likely contributor to limitation on regeneration. We have removed existing scar tissue in chronically contused rat spinal cord using a rose Bengal-based photo ablation approach. In this study, we compared two chemically modified rose bengal derivatives to unmodified rose bengal, both confirming and expanding on our previously published report. Rats were treated with unmodified rose bengal (RB1) or rose bengal modified with hydrocarbon (RB2) or polyethylene glycol (RB3), to determine the effects on scar components and spared tissue post-treatment. Our results showed that RB1 was more efficacious than RB2, while still maintaining minimal collateral effects on spared tissue. RB3 was not taken up by the cells, likely because of its size, and therefore had no effect. Treatment with RB1 also resulted in an increase in serotonin eight days post-treatment in chronically injured spinal cords. Thus, we suggest that unmodified rose Bengal is a potent candidate agent for the development of a therapeutic strategy for scar ablation in chronic SCI.

Original languageEnglish (US)
Pages (from-to)1745-1754
Number of pages10
JournalJournal of neurotrauma
Volume35
Issue number15
DOIs
StatePublished - Aug 1 2018

Fingerprint

Rose Bengal
Spinal Cord Injuries
Neuroglia
Cicatrix
Safety
Spinal Cord
Therapeutics
Contusions
Wounds and Injuries
Hydrocarbons
Regeneration
Serotonin
Animal Models

Keywords

  • chronic spinal cord injury
  • photochemical scar ablation
  • rose Bengal

Cite this

Safety and Efficacy of Rose Bengal Derivatives for Glial Scar Ablation in Chronic Spinal Cord Injury. / Patil, Nandadevi; Truong, Vincent; Holmberg, MacKenzie H.; Lavoie, Nicolas S.; McCoy, Mark R.; Dutton, James R; Holmberg, Eric G.; Parr, Ann M.

In: Journal of neurotrauma, Vol. 35, No. 15, 01.08.2018, p. 1745-1754.

Research output: Contribution to journalArticle

Patil, Nandadevi ; Truong, Vincent ; Holmberg, MacKenzie H. ; Lavoie, Nicolas S. ; McCoy, Mark R. ; Dutton, James R ; Holmberg, Eric G. ; Parr, Ann M. / Safety and Efficacy of Rose Bengal Derivatives for Glial Scar Ablation in Chronic Spinal Cord Injury. In: Journal of neurotrauma. 2018 ; Vol. 35, No. 15. pp. 1745-1754.
@article{c9510d19d34646438209f94b713a95c0,
title = "Safety and Efficacy of Rose Bengal Derivatives for Glial Scar Ablation in Chronic Spinal Cord Injury",
abstract = "There are no effective therapies available currently to ameliorate loss of function for patients with spinal cord injuries (SCIs). In addition, proposed treatments that demonstrated functional recovery in animal models of acute SCI have failed almost invariably when applied to chronic injury models. Glial scar formation in chronic injury is a likely contributor to limitation on regeneration. We have removed existing scar tissue in chronically contused rat spinal cord using a rose Bengal-based photo ablation approach. In this study, we compared two chemically modified rose bengal derivatives to unmodified rose bengal, both confirming and expanding on our previously published report. Rats were treated with unmodified rose bengal (RB1) or rose bengal modified with hydrocarbon (RB2) or polyethylene glycol (RB3), to determine the effects on scar components and spared tissue post-treatment. Our results showed that RB1 was more efficacious than RB2, while still maintaining minimal collateral effects on spared tissue. RB3 was not taken up by the cells, likely because of its size, and therefore had no effect. Treatment with RB1 also resulted in an increase in serotonin eight days post-treatment in chronically injured spinal cords. Thus, we suggest that unmodified rose Bengal is a potent candidate agent for the development of a therapeutic strategy for scar ablation in chronic SCI.",
keywords = "chronic spinal cord injury, photochemical scar ablation, rose Bengal",
author = "Nandadevi Patil and Vincent Truong and Holmberg, {MacKenzie H.} and Lavoie, {Nicolas S.} and McCoy, {Mark R.} and Dutton, {James R} and Holmberg, {Eric G.} and Parr, {Ann M}",
year = "2018",
month = "8",
day = "1",
doi = "10.1089/neu.2017.5398",
language = "English (US)",
volume = "35",
pages = "1745--1754",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "15",

}

TY - JOUR

T1 - Safety and Efficacy of Rose Bengal Derivatives for Glial Scar Ablation in Chronic Spinal Cord Injury

AU - Patil, Nandadevi

AU - Truong, Vincent

AU - Holmberg, MacKenzie H.

AU - Lavoie, Nicolas S.

AU - McCoy, Mark R.

AU - Dutton, James R

AU - Holmberg, Eric G.

AU - Parr, Ann M

PY - 2018/8/1

Y1 - 2018/8/1

N2 - There are no effective therapies available currently to ameliorate loss of function for patients with spinal cord injuries (SCIs). In addition, proposed treatments that demonstrated functional recovery in animal models of acute SCI have failed almost invariably when applied to chronic injury models. Glial scar formation in chronic injury is a likely contributor to limitation on regeneration. We have removed existing scar tissue in chronically contused rat spinal cord using a rose Bengal-based photo ablation approach. In this study, we compared two chemically modified rose bengal derivatives to unmodified rose bengal, both confirming and expanding on our previously published report. Rats were treated with unmodified rose bengal (RB1) or rose bengal modified with hydrocarbon (RB2) or polyethylene glycol (RB3), to determine the effects on scar components and spared tissue post-treatment. Our results showed that RB1 was more efficacious than RB2, while still maintaining minimal collateral effects on spared tissue. RB3 was not taken up by the cells, likely because of its size, and therefore had no effect. Treatment with RB1 also resulted in an increase in serotonin eight days post-treatment in chronically injured spinal cords. Thus, we suggest that unmodified rose Bengal is a potent candidate agent for the development of a therapeutic strategy for scar ablation in chronic SCI.

AB - There are no effective therapies available currently to ameliorate loss of function for patients with spinal cord injuries (SCIs). In addition, proposed treatments that demonstrated functional recovery in animal models of acute SCI have failed almost invariably when applied to chronic injury models. Glial scar formation in chronic injury is a likely contributor to limitation on regeneration. We have removed existing scar tissue in chronically contused rat spinal cord using a rose Bengal-based photo ablation approach. In this study, we compared two chemically modified rose bengal derivatives to unmodified rose bengal, both confirming and expanding on our previously published report. Rats were treated with unmodified rose bengal (RB1) or rose bengal modified with hydrocarbon (RB2) or polyethylene glycol (RB3), to determine the effects on scar components and spared tissue post-treatment. Our results showed that RB1 was more efficacious than RB2, while still maintaining minimal collateral effects on spared tissue. RB3 was not taken up by the cells, likely because of its size, and therefore had no effect. Treatment with RB1 also resulted in an increase in serotonin eight days post-treatment in chronically injured spinal cords. Thus, we suggest that unmodified rose Bengal is a potent candidate agent for the development of a therapeutic strategy for scar ablation in chronic SCI.

KW - chronic spinal cord injury

KW - photochemical scar ablation

KW - rose Bengal

UR - http://www.scopus.com/inward/record.url?scp=85049690787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049690787&partnerID=8YFLogxK

U2 - 10.1089/neu.2017.5398

DO - 10.1089/neu.2017.5398

M3 - Article

VL - 35

SP - 1745

EP - 1754

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 15

ER -