SadA, a novel adhesion receptor in Dictyostelium

Petra Fey, Stephen Stephens, Margaret A. Titus, Rex L. Chisholm

Research output: Contribution to journalArticlepeer-review

93 Scopus citations


Little is known about cell-substrate adhesion and how motile and adhesive forces work together in moving cells. The ability to rapidly screen a large number of insertional mutants prompted us to perform a genetic screen in Dictyostelium to isolate adhesion-deficient mutants. The resulting substrate adhesion-deficient (sad) mutants grew in plastic dishes without attaching to the substrate. The cells were often larger than their wild-type parents and displayed a rough surface with many apparent blebs. One of these mutants, sadA-, completely lacked substrate adhesion in growth medium. The sadA- mutant also showed slightly impaired cytokinesis, an aberrant F-actin organization, and a phagocytosis defect. Deletion of the sadA gene by homologous recombination recreated the original mutant phenotype. Expression of sadA-GFP in sadA-null cells restored the wild-type phenotype. In sadA-GFP-rescued mutant cells, sadA-GFP localized to the cell surface, appropriate for an adhesion molecule. SadA contains nine putative transmembrane domains and three conserved EGF-like repeats in a predicted extracellular domain. The EGF repeats are similar to corresponding regions in proteins known to be involved in adhesion, such as tenascins and integrins. Our data combined suggest that sadA is the first substrate adhesion receptor to be identified in Dictyostelium.

Original languageEnglish (US)
Pages (from-to)1109-1119
Number of pages11
JournalJournal of Cell Biology
Issue number6
StatePublished - Dec 23 2002


  • Cell-substrate adhesion
  • Cytokinesis
  • Dictyostelium
  • EGF-like repeats
  • Phagocytosis


Dive into the research topics of 'SadA, a novel adhesion receptor in Dictyostelium'. Together they form a unique fingerprint.

Cite this