One activity ascribed to Sac1p is the transport of ATP into the lumen of the endoplasmic reticulum of Saccharomyces cerevisiae; therefore, the question of whether this protein plays a role in ATP efflux from yeast was addressed. Preliminary results suggested that deletion of the SAC1 gene eliminated nigericin stimulated ATP efflux. However, further experimentation revealed that this result was caused by a pronounced extracellular ATPase activity for sac1Δ cells at alkaline pH, conditions required to measure extracellular ATP in wild type cells. At acid pH, sac1Δ cells exhibit glucose-dependent, nigericin-stimulated ATP efflux. sac1Δ cells express less acid phosphatase activity in the periplasm than do wild type cells, thus increasing the stability of extracellular ATP. At alkaline pH, however, sac1Δ cells tend to lose structural integrity and release lactate dehydrogenase as well as an unidentified ATPase activity to the extracellular fluid. Therefore, Sac1p is not involved in ATP efflux from S. cerevisiae.
|Original language||English (US)|
|Number of pages||4|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 9 1997|
Bibliographical noteFunding Information:
We thank Vytas Bankaitis for the yeast strains. This work was supported by NIH Grant HLO8893 to Guido Guidotti and a Predoctoral Fellowship from the Howard Hughes Medical Institute to Rodney Boyum.