Rpg7: A new gene for stem rust resistance from Hordeum vulgare ssp. spontaneum

Eva C Henningsen, Ahmad H. Sallam, Oadi Matny, Tamas Szinyei, Melania Figueroa, Brian J. Steffenson

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Wheat stem rust (causal organism: Puccinia graminis f. sp. tritici) is an important fungal disease that causes significant yield losses in barley. The deployment of resistant cultivars is the most effective means of controlling this disease. Stem rust evaluations of a diverse collection of wild barley (Hordeum vulgare ssp. spontaneum) identified two Jordanian accessions (WBDC094 and WBDC238) with resistance to a virulent pathotype (P. graminis f. sp. tritici HKHJC) from the United States. To elucidate the genetics of stem rust resistance, both accessions were crossed to the susceptible landrace Hiproly. Segregation ratios of F2 and F3 progeny indicated that a single dominant gene confers resistance to P. graminis f. sp. tritici HKHJC. Molecular mapping of the resistance locus was performed in the Hiproly/WBDC238 F2 population based on 3,329 single-nucleotide polymorphism markers generated by genotyping-by-sequencing. Quantitative trait locus analysis positioned the resistance gene to the long arm of chromosome 3H between the physical/genetic positions of 683.8 Mbp/172.9 cM and 693.7 Mbp/176.0 cM. Because this resistance gene is novel, it was assigned the new gene locus symbol of Rpg7 with a corresponding allele symbol of Rpg7.i. At the seedling stage, Rpg7 confers resistance against a number of other important P. graminis f. sp. tritici pathotypes from the United States (MCCFC, QCCJB, and TTTTF) and Africa (TTKSK) as well as an isolate (92-MN-90) of the rye stem rust pathogen (P. graminis f. sp. secalis) from Minnesota. The resistance conferred by Rpg7 can be readily transferred into breeding programs because of its simple inheritance and clear phenotypic expression.

Original languageEnglish (US)
Pages (from-to)548-558
Number of pages11
JournalPhytopathology
Volume111
Issue number3
DOIs
StatePublished - Mar 2021

Bibliographical note

Funding Information:
Funding: This research was supported in part by the USDA Agricultural Research Service Cooperative Agreement 58-5062-0-007 (Developing Stem Rust Resistant Barley Germplasm), Lieberman-Okinow Endowment at the University of Minnesota, State of Minnesota Small Grains Initiative, American Malting Barley Association Minnesota Agricultural Experiment Station, the National Institute of Food and Agriculture, U.S. Department of Agriculture [Hatch project MIN-22-085], and the University of Minnesota Undergraduate Research Opportunities Program.

Publisher Copyright:
© 2021 The American Phytopathological Society

Keywords

  • Puccinia graminis
  • Resistance mapping
  • Stem rust
  • Wild barley

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