ROS-mediated plasmodesmal regulation requires a network of an Arabidopsis receptor-like kinase, calmodulin-like proteins, and callose synthases

Minh Huy Vu, Tae Kyung Hyun, Sungwha Bahk, Yeonhwa Jo, Ritesh Kumar, Dhineshkumar Thiruppathi, Arya Bagus Boedi Iswanto, Woo Sik Chung, Rahul Mahadev Shelake, Jae Yean Kim

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Plasmodesmata (PD) play a critical role in symplasmic communication, coordinating plant activities related to growth & development, and environmental stress responses. Most developmental and environmental stress signals induce reactive oxygen species (ROS)-mediated signaling in the apoplast that causes PD closure by callose deposition. Although the apoplastic ROS signals are primarily perceived at the plasma membrane (PM) by receptor-like kinases (RLKs), such components involved in PD regulation are not yet known. Here, we show that an Arabidopsis NOVEL CYS-RICH RECEPTOR KINASE (NCRK), a PD-localized protein, is required for plasmodesmal callose deposition in response to ROS stress. We identified the involvement of NCRK in callose accumulation at PD channels in either basal level or ROS-dependent manner. Loss-of-function mutant (ncrk) of NCRK induces impaired callose accumulation at the PD under the ROS stress resembling a phenotype of the PD-regulating GLUCAN SYNTHASE-LIKE 4 (gsl4) knock-out plant. The overexpression of transgenic NCRK can complement the callose and the PD permeability phenotypes of ncrk mutants but not kinase-inactive NCRK variants or Cys-mutant NCRK, in which Cys residues were mutated in Cys-rich repeat ectodomain. Interestingly, NCRK mediates plasmodesmal permeability in mechanical injury-mediated signaling pathways regulated by GSL4. Furthermore, we show that NCRK interacts with calmodulin-like protein 41 (CML41) and GSL4 in response to ROS stress. Altogether, our data indicate that NCRK functions as an upstream regulator of PD callose accumulation in response to ROS-mediated stress signaling pathways.

Original languageEnglish (US)
Article number1107224
JournalFrontiers in Plant Science
Volume13
DOIs
StatePublished - Jan 19 2023

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (grants NRF 2022R1A2C3010331, 2021R1A5A8029490, 2020M3A9I4038352, and 2020R1A6A1A03044344).

Publisher Copyright:
Copyright © 2023 Vu, Hyun, Bahk, Jo, Kumar, Thiruppathi, Iswanto, Chung, Shelake and Kim.

Keywords

  • abiotic and biotic stress
  • callose
  • plasmodesmata
  • receptor-like kinase (RLK)
  • ROS perception

PubMed: MeSH publication types

  • Journal Article

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