ROOT ULTRAVIOLET B-SENSITIVE1/WEAK AUXIN RESPONSE3 is essential for polar auxin transport in Arabidopsis

Hong Yu, Michael Karampelias, Stephanie Robert, Wendy Ann Peer, Ranjan Swarup, Songqing Ye, Lei Ge, Jerry Cohen, Angus Murphy, Jirí Friml, Mark Estelle

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The phytohormone auxin regulates virtually every aspect of plant development. To identify new genes involved in auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases polar auxin transport and results in a disruption of the asymmetric auxin distribution. The levels of the auxin transporters AUXIN1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of polar auxin transport by maintaining the proper level of auxin transporters on the plasma membrane.

Original languageEnglish (US)
Pages (from-to)965-976
Number of pages12
JournalPlant physiology
Volume162
Issue number2
DOIs
StatePublished - Jun 2013

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