Roles of Arenavirus Z Protein in Mediating Virion Budding, Viral Transcription-Inhibition and Interferon-Beta Suppression

Junjie Shao, Yuying Liang, Hinh Ly

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The smallest arenaviral protein is the zinc-finger protein (Z) that belongs to the RING finger protein family. Z serves as a main component required for virus budding from the membrane of the infected cells through self-oligomerization, a process that can be aided by the viral nucleoprotein (NP) to form the viral matrix of progeny virus particles. Z has also been shown to be essential for mediating viral transcriptional repression activity by locking the L polymerase onto the viral promoter in a catalytically inactive state, thus limiting viral replication. The Z protein has also recently been shown to inhibit the type I interferon-induction pathway by directly binding to the intracellular pathogen-sensor proteins RIG-I and MDA5, and thus inhibiting their normal functions. This chapter describes several assays used to examine the important roles of the arenaviral Z protein in mediating virus budding (i.e., either Z self-budding or NP-Z budding activities), viral transcriptional inhibition in a viral minigenome (MG) assay, and type I IFN suppression in an IFN-β promoter-mediated luciferase reporter assay.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages217-227
Number of pages11
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1604
ISSN (Print)1064-3745

Keywords

  • Arenavirus
  • Interferon suppression
  • Lassa virus
  • Matrix protein
  • Pichindé virus
  • RING protein
  • Transcription inhibition
  • Virus budding

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    Shao, J., Liang, Y., & Ly, H. (2018). Roles of Arenavirus Z Protein in Mediating Virion Budding, Viral Transcription-Inhibition and Interferon-Beta Suppression. In Methods in Molecular Biology (pp. 217-227). (Methods in Molecular Biology; Vol. 1604). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6981-4_16