Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis

Sakai Hiroshi, Hiasa Hiroshi, Iwamoto Keiji, Horimoto Taizo, Komano Tohru, Godson G. Nigel

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Phage G4 origin of complementary DNA strand synthesis (oric) consists of three stable stem-loop structures (I, II, and III). Mutant oric sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II-III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo oric function. The results suggest that recognition sequences for dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.

Original languageEnglish (US)
Pages (from-to)323-330
Number of pages8
JournalGene
Volume71
Issue number2
DOIs
StatePublished - Nov 30 1988

Bibliographical note

Funding Information:
We thank Dr. N. Nomura for providingu s with bacteriaal ndp hages trains,a ndDr. H. Ohkawaf or providingu s with syntheticD NAs. This work was fundedb y PublicH ealthS erviceG rant GM38292t o G.N.G.

Keywords

  • Bacteriophage genome replication
  • M13 phage vector
  • RNA primer
  • cloned ori
  • dnaG primase
  • mutants
  • recombinant DNA
  • stem-loop structures

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