Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis

Sakai Hiroshi; Hiasa Hiroshi; Iwamoto Keiji; Horimoto Taizo; Komano Tohru; Godson G. Nigel

doi:10.1016/0378-1119(88)90049-2

Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis

Sakai Hiroshi, Hiasa Hiroshi, Iwamoto Keiji, Horimoto Taizo, Komano Tohru, Godson G. Nigel

Pharmacology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Phage G4 origin of complementary DNA strand synthesis (ori_c) consists of three stable stem-loop structures (I, II, and III). Mutant ori_c sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II-III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo ori_c function. The results suggest that recognition sequences for dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.

Original language	English (US)
Pages (from-to)	323-330
Number of pages	8
Journal	Gene
Volume	71
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(88)90049-2
State	Published - Nov 30 1988

Bibliographical note

Funding Information:
We thank Dr. N. Nomura for providingu s with bacteriaal ndp hages trains,a ndDr. H. Ohkawaf or providingu s with syntheticD NAs. This work was fundedb y PublicH ealthS erviceG rant GM38292t o G.N.G.

Keywords

Bacteriophage genome replication
M13 phage vector
RNA primer
cloned ori
dnaG primase
mutants
recombinant DNA
stem-loop structures

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10.1016/0378-1119(88)90049-2

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title = "Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis",

abstract = "Phage G4 origin of complementary DNA strand synthesis (oric) consists of three stable stem-loop structures (I, II, and III). Mutant oric sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II-III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo oric function. The results suggest that recognition sequences for dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.",

keywords = "Bacteriophage genome replication, M13 phage vector, RNA primer, cloned ori, dnaG primase, mutants, recombinant DNA, stem-loop structures",

author = "Sakai Hiroshi and Hiasa Hiroshi and Iwamoto Keiji and Horimoto Taizo and Komano Tohru and {G. Nigel}, Godson",

note = "Funding Information: We thank Dr. N. Nomura for providingu s with bacteriaal ndp hages trains,a ndDr. H. Ohkawaf or providingu s with syntheticD NAs. This work was fundedb y PublicH ealthS erviceG rant GM38292t o G.N.G.",

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doi = "10.1016/0378-1119(88)90049-2",

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T1 - Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis

AU - Hiroshi, Sakai

AU - Hiroshi, Hiasa

AU - Keiji, Iwamoto

AU - Taizo, Horimoto

AU - Tohru, Komano

AU - G. Nigel, Godson

N1 - Funding Information: We thank Dr. N. Nomura for providingu s with bacteriaal ndp hages trains,a ndDr. H. Ohkawaf or providingu s with syntheticD NAs. This work was fundedb y PublicH ealthS erviceG rant GM38292t o G.N.G.

PY - 1988/11/30

Y1 - 1988/11/30

N2 - Phage G4 origin of complementary DNA strand synthesis (oric) consists of three stable stem-loop structures (I, II, and III). Mutant oric sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II-III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo oric function. The results suggest that recognition sequences for dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.

AB - Phage G4 origin of complementary DNA strand synthesis (oric) consists of three stable stem-loop structures (I, II, and III). Mutant oric sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II-III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo oric function. The results suggest that recognition sequences for dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.

KW - Bacteriophage genome replication

KW - M13 phage vector

KW - RNA primer

KW - cloned ori

KW - dnaG primase

KW - mutants

KW - recombinant DNA

KW - stem-loop structures

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JO - Gene

JF - Gene

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ER -

Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis

Abstract

Bibliographical note

Keywords

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