Role of the Macrophage in the Translocation of Intestinal Bacteria

Carol L. Wells, Michael A. Maddaus, Richard L. Simmons

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

To clarify the role of the macrophage in the translocation of intestinal bacteria, groups (n = 10) of Swiss Webster mice (immunocompetent) and C3H/HeJ mice (macrophage defective) were given bacitracin/streptomycin in their drinking water to eliminate the majority of the intestinal microflora. These mice were then “monoassociated” with a streptomycin-resistant strain of Escherichia coli. Forty-eight hours later, E coli was present in all animals at a concentration of 1011/g of cecum. In four separate experiments, E coli was recovered from 100% of the mesenteric lymph nodes (MLNs) of the immunocompetent Swiss Webster mice and from 10%, 40%, 30%, and 50% of the MLNs of macrophage-defective C3H/HeJ mice. Swiss Webster mice were then similarly monoassociated by antibiotic decontamination followed by administration of antibiotic-resistant, fluorescein-labeled E coli in their drinking water; cohort groups of mice were given fluorescein-labeled latex beads (1 μm in diameter) in their drinking water. Two, four, and 11 days later, the MLNs were removed and single cell suspensions were analyzed in the fluorescence-activated cell sorter. The fluorescein label was detected exclusively in the macrophage (esterase-positive) population. These results support the hypothesis that intestinal macrophages may play a key role in the transport of intestinal particles (including bacteria) into extra-intestinal sites.

Original languageEnglish (US)
Pages (from-to)48-53
Number of pages6
JournalArchives of Surgery
Volume122
Issue number1
DOIs
StatePublished - Jan 1987

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