Role of the far-upstream sites of the algD promoter and the algR and rpoN genes in environmental modulation of mucoidy in Pseudomonas aeruginosa

C. D. Mohr, D. W. Martin, W. M. Konyecsni, J. R.W. Govan, S. Lory, V. Deretic

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Abstract

The role of several regulatory elements in environmental modulation of mucoidy in Pseudomonas aeruginosa was studied. Transcriptional activation of algD, necessary for the mucoid phenotype, was found to depend on FUS, the newly identified far-upstream sites of the algD promoter. The FUS were delimited to a region spanning nucleotides -432 to -332 relative to the algD mRNA start site. Insertional inactivation of algR in PAO568 abolished the algD promoter response to nitrogen availability and greatly diminished but did not completely eliminate reactivity to changes in salt concentration. Insertional inactivation of rpoN (ntrA) in PAO568 did not affect algR and algD transcription.

Original languageEnglish (US)
Pages (from-to)6576-6580
Number of pages5
JournalJournal of bacteriology
Volume172
Issue number11
DOIs
StatePublished - 1990

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