Role of the B component of methylosinus trichosporium OB3B methane monooxygenase

Research output: Contribution to journalConference article

Abstract

Methane monooxygenase (MMO) is a three-component enzyme that catalyzes the reaction of methane with oxygen to form methanol. Through site-directed mutagenesis and spectroscopies, the disordered C-terminus of MMOB is shown to be required for efficient catalysis and the formation of a key peroxo intermediate or its precursor. The deletion of the entire C-terminal segment perturbed the electronic environment of the active site in the hydroxylase component (MMOH), suggesting that C-terminus is likely to be located in or near the interaction interfaces between MMOH and MMOB. This is an abstract of a paper presented at the 228th ACS National Meeting (Philadelphia, PA 8/22-26/2004).

Original languageEnglish (US)
JournalACS National Meeting Book of Abstracts
Volume228
Issue number1
StatePublished - Oct 20 2004
EventAbstracts of Papers - 228th ACS National Meeting - Philadelphia, PA, United States
Duration: Aug 22 2004Aug 26 2004

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methane monooxygenase
Mutagenesis
Methane
Mixed Function Oxygenases
Catalysis
Methanol
Spectroscopy
Oxygen
Enzymes

Cite this

Role of the B component of methylosinus trichosporium OB3B methane monooxygenase. / Zhang, Jingyan; Lipscomb, John D.

In: ACS National Meeting Book of Abstracts, Vol. 228, No. 1, 20.10.2004.

Research output: Contribution to journalConference article

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AU - Lipscomb, John D

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N2 - Methane monooxygenase (MMO) is a three-component enzyme that catalyzes the reaction of methane with oxygen to form methanol. Through site-directed mutagenesis and spectroscopies, the disordered C-terminus of MMOB is shown to be required for efficient catalysis and the formation of a key peroxo intermediate or its precursor. The deletion of the entire C-terminal segment perturbed the electronic environment of the active site in the hydroxylase component (MMOH), suggesting that C-terminus is likely to be located in or near the interaction interfaces between MMOH and MMOB. This is an abstract of a paper presented at the 228th ACS National Meeting (Philadelphia, PA 8/22-26/2004).

AB - Methane monooxygenase (MMO) is a three-component enzyme that catalyzes the reaction of methane with oxygen to form methanol. Through site-directed mutagenesis and spectroscopies, the disordered C-terminus of MMOB is shown to be required for efficient catalysis and the formation of a key peroxo intermediate or its precursor. The deletion of the entire C-terminal segment perturbed the electronic environment of the active site in the hydroxylase component (MMOH), suggesting that C-terminus is likely to be located in or near the interaction interfaces between MMOH and MMOB. This is an abstract of a paper presented at the 228th ACS National Meeting (Philadelphia, PA 8/22-26/2004).

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