Role of substrate in imparting calcium and phospholipid requirements to protein kinase C activation

Mohammad D. Bazzi, Gary L. Nelsestuen

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140 Scopus citations

Abstract

The role of substrate in influencing the cofactor requirements of the phospholipid- and Ca2+-dependent protein kinase C (PKC) was investigated by using several substrates. All of the substrates tested, including histone, troponin I, myosin light chain, protamine, poly(arginine, serine) (PAS), poly(lysine, serine) (PLS), and myelin basic protein (MBP), were found to interact with and aggregate phospholipid vesicles as well as phosphatidylserine (PS)-Triton mixed micelles. Phosphorylation of these different substrates by PKC indicated the presence of three distinct substrate categories: (A) substrates such as protamine requiring no cofactors; (B) substrates such as PLS, PAS, and MBP requiring only the presence of phospholipid; and (C) substrates such as histone, myosin light chain, and troponin I requiring the presence of Ca2+ and phospholipid. Diacylglycerol was a major cofactor only with category C substrates. These different requirements correlated with the interaction of the substrate with phospholipid and/or enzyme. The substrates in category A interacted strongly with and aggregated PKC in a binary mixture. In the absence of Ca2+, PKC bound to substrates of category B directly but not to substrates in category C. Thus, substrate-enzyme binding eliminated the Ca2+ requirement of phosphorylation, and aggregation of substrate-enzyme complex eliminated the phospholipid requirements as well. Substrate-phospholipid interaction and substrate phosphorylation were inhibited by increasing salt concentrations, but the amount needed depended upon the substrate. Loss of PKC activity appeared to coincide with loss of substrate-PS aggregation while dissociation of PKC from the membranes required much higher salt concentrations. Poly(L-lysine) and poly(L-arginine), two potent inhibitors of PKC, also showed substrate-dependent inhibition characteristics. Both of these polymers interacted strongly with phospholipid vesicles and appeared to exert their inhibition by interfering with substrate-phospholipid binding. These studies indicated that the substrate plays an important role in imparting Ca2+, diacylglycerol, and phospholipid requirements to the activation of PKC, and titrations of PKC activity with phospholipid were actually dominated by substrate-phospholipid interactions. Delivery of substrate to the active site appears to be a key event in PKC phosphorylation, and this process may exhibit a requirement for phospholipid and/or Ca2+ depending upon the choice of substrate.

Original languageEnglish (US)
Pages (from-to)1974-1982
Number of pages9
JournalBiochemistry
Volume26
Issue number7
StatePublished - 1987

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