Role of cytokines in lipopolysaccharide‐induced functional and structural abnormalities of astrocytes

Shuxian Hu; Alice Martella; W. Robert Anderson; Chun C. Chao

doi:10.1002/glia.440100309

Role of cytokines in lipopolysaccharide‐induced functional and structural abnormalities of astrocytes

Shuxian Hu, Alice Martella, W. Robert Anderson, Chun C. Chao

Medicine - Infectious Disease and International Medicine

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The mechanism underlying meningitis‐associated brain injury is unclear. This study investigated the hypothesis that lipopolysaccharide (LPS) alters astrocyte function and structure via the release of proinflammatory cytokines. In enriched murine astrocyte cultures, LPS inhibited (P < 0.05) glutamine synthetase activity, ³H‐gamma aminobutyric acid uptake, and DNA synthesis; LPS also induced ultrastructural changes. Antibodies to tumor necrosis factor‐α, interleukin‐1, and interleukin‐6 blocked (P < 0.05) in part the LPS‐induced inhibition of astrocyte function. Also, treatment of astrocyte cultures with cytokines significantly altered these astrocyte functions and ultrastructure. Taken together, the present findings support the hypothesis that LPS affects astrocyte function and structure via the release of proinflammatory cytokines, especially tumor necrosis factor‐α. © 1994 Wiley‐Liss, Inc.

Original language	English (US)
Pages (from-to)	227-234
Number of pages	8
Journal	Glia
Volume	10
Issue number	3
DOIs	https://doi.org/10.1002/glia.440100309
State	Published - Mar 1994

Keywords

GABA uptake
Glutamine synthetase
Interleukin‐1
Interleukin‐6
Tumor necrosis factor‐α

Publisher link

10.1002/glia.440100309

Find It

Find It at U of M Libraries

Cite this

@article{d6ede00d7ef9472986bfb02ad0b90eef,

title = "Role of cytokines in lipopolysaccharide‐induced functional and structural abnormalities of astrocytes",

abstract = "The mechanism underlying meningitis‐associated brain injury is unclear. This study investigated the hypothesis that lipopolysaccharide (LPS) alters astrocyte function and structure via the release of proinflammatory cytokines. In enriched murine astrocyte cultures, LPS inhibited (P < 0.05) glutamine synthetase activity, 3H‐gamma aminobutyric acid uptake, and DNA synthesis; LPS also induced ultrastructural changes. Antibodies to tumor necrosis factor‐α, interleukin‐1, and interleukin‐6 blocked (P < 0.05) in part the LPS‐induced inhibition of astrocyte function. Also, treatment of astrocyte cultures with cytokines significantly altered these astrocyte functions and ultrastructure. Taken together, the present findings support the hypothesis that LPS affects astrocyte function and structure via the release of proinflammatory cytokines, especially tumor necrosis factor‐α. {\textcopyright} 1994 Wiley‐Liss, Inc.",

keywords = "GABA uptake, Glutamine synthetase, Interleukin‐1, Interleukin‐6, Tumor necrosis factor‐α",

author = "Shuxian Hu and Alice Martella and Anderson, {W. Robert} and Chao, {Chun C.}",

year = "1994",

month = mar,

doi = "10.1002/glia.440100309",

language = "English (US)",

volume = "10",

pages = "227--234",

journal = "Glia",

issn = "0894-1491",

publisher = "John Wiley and Sons Inc.",

number = "3",

}

TY - JOUR

T1 - Role of cytokines in lipopolysaccharide‐induced functional and structural abnormalities of astrocytes

AU - Hu, Shuxian

AU - Martella, Alice

AU - Anderson, W. Robert

AU - Chao, Chun C.

PY - 1994/3

Y1 - 1994/3

N2 - The mechanism underlying meningitis‐associated brain injury is unclear. This study investigated the hypothesis that lipopolysaccharide (LPS) alters astrocyte function and structure via the release of proinflammatory cytokines. In enriched murine astrocyte cultures, LPS inhibited (P < 0.05) glutamine synthetase activity, 3H‐gamma aminobutyric acid uptake, and DNA synthesis; LPS also induced ultrastructural changes. Antibodies to tumor necrosis factor‐α, interleukin‐1, and interleukin‐6 blocked (P < 0.05) in part the LPS‐induced inhibition of astrocyte function. Also, treatment of astrocyte cultures with cytokines significantly altered these astrocyte functions and ultrastructure. Taken together, the present findings support the hypothesis that LPS affects astrocyte function and structure via the release of proinflammatory cytokines, especially tumor necrosis factor‐α. © 1994 Wiley‐Liss, Inc.

AB - The mechanism underlying meningitis‐associated brain injury is unclear. This study investigated the hypothesis that lipopolysaccharide (LPS) alters astrocyte function and structure via the release of proinflammatory cytokines. In enriched murine astrocyte cultures, LPS inhibited (P < 0.05) glutamine synthetase activity, 3H‐gamma aminobutyric acid uptake, and DNA synthesis; LPS also induced ultrastructural changes. Antibodies to tumor necrosis factor‐α, interleukin‐1, and interleukin‐6 blocked (P < 0.05) in part the LPS‐induced inhibition of astrocyte function. Also, treatment of astrocyte cultures with cytokines significantly altered these astrocyte functions and ultrastructure. Taken together, the present findings support the hypothesis that LPS affects astrocyte function and structure via the release of proinflammatory cytokines, especially tumor necrosis factor‐α. © 1994 Wiley‐Liss, Inc.

KW - GABA uptake

KW - Glutamine synthetase

KW - Interleukin‐1

KW - Interleukin‐6

KW - Tumor necrosis factor‐α

UR - http://www.scopus.com/inward/record.url?scp=0028389345&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028389345&partnerID=8YFLogxK

U2 - 10.1002/glia.440100309

DO - 10.1002/glia.440100309

M3 - Article

C2 - 7910808

AN - SCOPUS:0028389345

SN - 0894-1491

VL - 10

SP - 227

EP - 234

JO - Glia

JF - Glia

IS - 3

ER -

Role of cytokines in lipopolysaccharide‐induced functional and structural abnormalities of astrocytes

Abstract

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this